Regional and age-related diversity of human mature oligodendrocytes.

Autor: Yaqubi M; Neuro-immunology Unit, Montreal Neurological Institute and Department of Neurology and Neurosurgery, McGill University, Montreal, Canada., Luo JXX; Neuro-immunology Unit, Montreal Neurological Institute and Department of Neurology and Neurosurgery, McGill University, Montreal, Canada., Baig S; Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada., Cui QL; Neuro-immunology Unit, Montreal Neurological Institute and Department of Neurology and Neurosurgery, McGill University, Montreal, Canada., Petrecca K; Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada., Desu H; Department of Neurosciences, Centre de Recherche du Centre Hospitalier de l'Université deMontréal, Montreal, QC, Canada., Larochelle C; Department of Neurosciences, Centre de Recherche du Centre Hospitalier de l'Université deMontréal, Montreal, QC, Canada., Afanasiev E; Neuro-immunology Unit, Montreal Neurological Institute and Department of Neurology and Neurosurgery, McGill University, Montreal, Canada., Hall JA; Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada., Dudley R; Department of Pediatric Neurosurgery, Montreal Children's Hospital, Montreal, Canada., Srour M; Division of Pediatric Neurology, Montreal Children's Hospital, Montreal, Canada., Haglund L; The Orthopedic Research Laboratory, Department of Surgery, McGill University, Montreal, Canada., Ouellet J; The Orthopedic Research Laboratory, Department of Surgery, McGill University, Montreal, Canada.; McGill Scoliosis and Spine Group, Department of Surgery, McGill University, Montreal, Canada., Georgiopoulos M; Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada.; The Orthopedic Research Laboratory, Department of Surgery, McGill University, Montreal, Canada., Santaguida C; McGill Scoliosis and Spine Group, Department of Surgery, McGill University, Montreal, Canada., Sonnen JA; Departments of Pathology, Neurology and Neurosurgery, McGill University, Montreal, QC, Canada., Healy LM; Neuro-immunology Unit, Montreal Neurological Institute and Department of Neurology and Neurosurgery, McGill University, Montreal, Canada., Stratton JA; Neuro-immunology Unit, Montreal Neurological Institute and Department of Neurology and Neurosurgery, McGill University, Montreal, Canada., Kennedy TE; Neuro-immunology Unit, Montreal Neurological Institute and Department of Neurology and Neurosurgery, McGill University, Montreal, Canada.; Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, Canada., Antel JP; Neuro-immunology Unit, Montreal Neurological Institute and Department of Neurology and Neurosurgery, McGill University, Montreal, Canada.
Jazyk: angličtina
Zdroj: Glia [Glia] 2022 Oct; Vol. 70 (10), pp. 1938-1949. Date of Electronic Publication: 2022 Jun 23.
DOI: 10.1002/glia.24230
Abstrakt: Morphological and emerging molecular studies have provided evidence for heterogeneity within the oligodendrocyte population. To address the regional and age-related heterogeneity of human mature oligodendrocytes (MOLs) we applied single-cell RNA sequencing to cells isolated from cortical/subcortical, subventricular zone brain tissue samples, and thoracolumbar spinal cord samples. Unsupervised clustering of cells identified transcriptionally distinct MOL subpopulations across regions. Spinal cord MOLs, but not microglia, exhibited cell-type-specific upregulation of immune-related markers compared to the other adult regions. SVZ MOLs showed an upregulation of select number of development-linked transcription factors compared to other regions; however, pseudotime trajectory analyses did not identify a global developmental difference. Age-related analysis of cortical/subcortical samples indicated that pediatric MOLs, especially from under age 5, retain higher expression of genes linked to development and to immune activity with pseudotime analysis favoring a distinct developmental stage. Our regional and age-related studies indicate heterogeneity of MOL populations in the human CNS that may reflect developmental and environmental influences.
(© 2022 Wiley Periodicals LLC.)
Databáze: MEDLINE
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