Exploring Honeybee Abdominal Anatomy through Micro-CT and Novel Multi-Staining Approaches.

Autor: De Paula JC; Grupo de Bioquímica y Parasitología Molecular CTS-183, Departamento de Parasitología, Universidad de Granada, 18071 Granada, Spain.; Institute of Biotechnology, Faculty of Sciences, University of Granada, 18071 Granada, Spain., Doello K; Medical Oncology Service, Virgen de las Nieves Hospital, 18014 Granada, Spain.; Instituto Biosanitario de Granada (ibs. GRANADA), 18014 Granada, Spain., Mesas C; Instituto Biosanitario de Granada (ibs. GRANADA), 18014 Granada, Spain.; Center of Biomedical Research (CIBM), Institute of Biopathology and Regenerative Medicine (IBIMER), University of Granada, 18100 Granada, Spain., Kapravelou G; Department of Physiology, Institute of Nutrition and Food Technology (INyTA), Biomedical Research Center (CIBM), Universidad de Granada, Avda del Conocimiento s/n, 18100 Granada, Spain., Cornet-Gómez A; Grupo de Bioquímica y Parasitología Molecular CTS-183, Departamento de Parasitología, Universidad de Granada, 18071 Granada, Spain.; Institute of Biotechnology, Faculty of Sciences, University of Granada, 18071 Granada, Spain., Orantes FJ; Apinevada S.L Parque Metropolitano Industrial de Granada, Calle Rubiales 17, 18130 Granada, Spain., Martínez R; Department of Physiology, Institute of Nutrition and Food Technology (INyTA), Biomedical Research Center (CIBM), Universidad de Granada, Avda del Conocimiento s/n, 18100 Granada, Spain., Linares F; Unidad de Microscopía de Fuerza Atómica, Centro de Instrumentación Científica, Universidad de Granada, 18003 Granada, Spain., Prados JC; Instituto Biosanitario de Granada (ibs. GRANADA), 18014 Granada, Spain.; Center of Biomedical Research (CIBM), Institute of Biopathology and Regenerative Medicine (IBIMER), University of Granada, 18100 Granada, Spain., Porres JM; Department of Physiology, Institute of Nutrition and Food Technology (INyTA), Biomedical Research Center (CIBM), Universidad de Granada, Avda del Conocimiento s/n, 18100 Granada, Spain., Osuna A; Grupo de Bioquímica y Parasitología Molecular CTS-183, Departamento de Parasitología, Universidad de Granada, 18071 Granada, Spain.; Institute of Biotechnology, Faculty of Sciences, University of Granada, 18071 Granada, Spain., de Pablos LM; Grupo de Bioquímica y Parasitología Molecular CTS-183, Departamento de Parasitología, Universidad de Granada, 18071 Granada, Spain.; Institute of Biotechnology, Faculty of Sciences, University of Granada, 18071 Granada, Spain.
Jazyk: angličtina
Zdroj: Insects [Insects] 2022 Jun 18; Vol. 13 (6). Date of Electronic Publication: 2022 Jun 18.
DOI: 10.3390/insects13060556
Abstrakt: Continuous improvements in morphological and histochemical analyses of Apis mellifera could improve our understanding of the anatomy and physiology of these insects at both the cellular and tissue level. In this work, two different approaches have been performed to add new data on the abdomen of worker bees: (i) Micro-computed tomography (Micro-CT), which allows the identification of small-scale structures (micrometers) with adequate/optimal resolution and avoids sample damage and, (ii) histochemical multi-staining with Periodic Acid-Schiff-Alcian blue, Lactophenol-Saphranin O and pentachrome staining to precisely characterize the histological structures of the midgut and hindgut. Micro-CT allowed high-resolution imaging of anatomical structures of the honeybee abdomen with particular emphasis on the proventriculus and pyloric valves, as well as the connection of the sting apparatus with the terminal abdominal ganglia. Furthermore, the histochemical analyses have allowed for the first-time description of ventricular telocytes in honeybees, a cell type located underneath the midgut epithelium characterized by thin and long cytoplasmic projections called telopodes. Overall, the analysis of these images could help the detailed anatomical description of the cryptic structures of honeybees and also the characterization of changes due to abiotic or biotic stress conditions.
Databáze: MEDLINE
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