Inhibition of SARS-CoV-2 spike protein entry using biologically modified polyacrylonitrile nanofibers: in vitro study towards specific antiviral masks.
| Autor: | Emam MH; Nanotechnology Research Center (NTRC), The British University in Egypt El-Shorouk City, Suez Desert Road, P.O. Box 43 Cairo 11837 Egypt Amal.Kasry@bue.edu.eg a.kasry@unesco.org., Nageh H; Nanotechnology Research Center (NTRC), The British University in Egypt El-Shorouk City, Suez Desert Road, P.O. Box 43 Cairo 11837 Egypt Amal.Kasry@bue.edu.eg a.kasry@unesco.org., Ali F; Nanotechnology Research Center (NTRC), The British University in Egypt El-Shorouk City, Suez Desert Road, P.O. Box 43 Cairo 11837 Egypt Amal.Kasry@bue.edu.eg a.kasry@unesco.org., Taha M; Nano Gate 9254 Hodashaarawy, Al Abageyah, El Mukkatam Cairo 43511 Egypt., ElShehaby HA; Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University Egypt., Amin R; Nano Gate 9254 Hodashaarawy, Al Abageyah, El Mukkatam Cairo 43511 Egypt.; National Institute of Laser Enhanced Science (NILES), Cairo University Giza 12613 Egypt., Kamoun EA; Nanotechnology Research Center (NTRC), The British University in Egypt El-Shorouk City, Suez Desert Road, P.O. Box 43 Cairo 11837 Egypt Amal.Kasry@bue.edu.eg a.kasry@unesco.org.; Polymeric Materials Research Dep., Advanced Technology and New Materials Research Institute (ATNMRI), City of Scientific Research and Technological Applications (SRTA-City) New Borg Al-Arab City 21934 Alexandria Egypt., Loutfy SA; Nanotechnology Research Center (NTRC), The British University in Egypt El-Shorouk City, Suez Desert Road, P.O. Box 43 Cairo 11837 Egypt Amal.Kasry@bue.edu.eg a.kasry@unesco.org.; Virology and Immunology Unit, Cancer Biology Department, National Cancer Institute, Cairo University Egypt., Kasry A; Nanotechnology Research Center (NTRC), The British University in Egypt El-Shorouk City, Suez Desert Road, P.O. Box 43 Cairo 11837 Egypt Amal.Kasry@bue.edu.eg a.kasry@unesco.org. |
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| Jazyk: | angličtina |
| Zdroj: | RSC advances [RSC Adv] 2022 May 31; Vol. 12 (25), pp. 16184-16193. Date of Electronic Publication: 2022 May 31 (Print Publication: 2022). |
| DOI: | 10.1039/d2ra01321e |
| Abstrakt: | With the increase of the contagiousness rates of Coronavirus disease (COVID-19), new strategies are needed to halt virus spread. Blocking virus entry by capturing its spike (S) protein is one of the effective approaches that could help in eliminating or reducing transmission rate of viruses. Herein, we aim to develop a nanofiber-based filter for protective face masks, composed of polyacrylonitrile (PAN) nanofibers (NFs)-loaded with Angiotensin Converting Enzyme-2 (ACE-2) for capturing the spike protein of severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) and blocking its entry. Docking simulations were performed to evaluate interactions of PAN with target proteins of both SARS-CoV-2 and Human Adenovirus type 5 (ADV-5) which was used as an in vitro model of human respiratory viruses. Scanning electron microscopy (SEM) and Fourier transformed infrared (FT-IR) spectroscopy was employed to investigate the surface morphology and to analyze the functional groups of the NFs, respectively. The mechanical properties of the electrospun NFs were investigated, according to which the tensile strengths of PAN and modified PAN NFs were 4.9 ± 1.2 GPa and 4.5 GPa. Additionally, elongations at break were 25 ± 2.5% to 24 ± 1.48% for PAN and modified PAN NFs. The tensile strength test showed good mechanical characteristics of the NFs. The ACE-2-loaded NFs were shown to be safe, with promising antiviral activity towards ADV-5. Meanwhile, a binding affinity study between the spike protein and ACE-2 was performed and the dissociation constant ( K Competing Interests: The authors declare no competing financial interest. (This journal is © The Royal Society of Chemistry.) |
| Databáze: | MEDLINE |
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