Principles and Problems of Exosome Isolation from Biological Fluids.

Autor: Yakubovich EI; Granov Russian Research Center for Radiology and Surgical Technologies, Ministry of Health of the Russian Federation, 197758 St. Petersburg, Russia., Polischouk AG; Granov Russian Research Center for Radiology and Surgical Technologies, Ministry of Health of the Russian Federation, 197758 St. Petersburg, Russia., Evtushenko VI; Granov Russian Research Center for Radiology and Surgical Technologies, Ministry of Health of the Russian Federation, 197758 St. Petersburg, Russia.
Jazyk: angličtina
Zdroj: Biochemistry (Moscow) Supplement. Series A, Membrane and cell biology [Biochem (Mosc) Suppl Ser A Membr Cell Biol] 2022; Vol. 16 (2), pp. 115-126. Date of Electronic Publication: 2022 Jun 16.
DOI: 10.1134/S1990747822030096
Abstrakt: Exosomes, the subclass of small membrane extracellular vesicles, have great diagnostic and therapeutic potential, but the lack of standardized methods for their efficient isolation and analysis limits the introduction of exosomal technologies into clinical practice. This review discusses the problems associated with the isolation of exosomes from biological fluids, as well as the principles of traditional and alternative methods of isolation. The aim of the presented review is to illustrate the variety of approaches based on the physical and biochemical properties of exosomes that can be used for exosome isolation. The advantages and disadvantages of different methods are discussed.
Competing Interests: The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
(© Pleiades Publishing, Ltd. 2022, ISSN 1990-7478, Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology, 2022, Vol. 16, No. 2, pp. 115–126. © Pleiades Publishing, Ltd., 2022.Russian Text © The Author(s), 2022, published in Biologicheskie Membrany, 2022, Vol. 39, No. 3, pp. 172–185.)
Databáze: MEDLINE
Externí odkaz: