Preservation of Adrenoceptor and Endothelin Receptor Mediated Vasoconstriction and of Endothelium-Dependent Relaxation after Cold Storage of Explanted Blood Vessels for ex vivo Analyses.

Autor:	Hoenicka M; Department of Cardiothoracic and Vascular Surgery, Ulm University Medical Center, Ulm, Germany., Sabau M; Department of Cardiothoracic and Vascular Surgery, Ulm University Medical Center, Ulm, Germany., Liebold A; Department of Cardiothoracic and Vascular Surgery, Ulm University Medical Center, Ulm, Germany., Hofmann HS; Department of Thoracic Surgery, University Medical Center Regensburg, Regensburg, Germany., Ried M; Department of Thoracic Surgery, University Medical Center Regensburg, Regensburg, Germany.
Jazyk:	angličtina
Zdroj:	Journal of vascular research [J Vasc Res] 2022; Vol. 59 (5), pp. 303-313. Date of Electronic Publication: 2022 Jun 21.
DOI:	10.1159/000524922
Abstrakt:	Introduction: Adrenoceptor and endothelin (ET) receptor-mediated vasoconstriction as well as endothelium-dependent vasodilation of human saphenous veins were compared before and after 20 h of cold storage. Methods: Contractile responses to potassium chloride (KCl), norepinephrine (NE), and ET-1 as well as vasodilator responses to acetylcholine (ACh) were evaluated. Results: Storage in HEPES-supplemented Dulbecco's modified Eagle's medium (HDMEM) diminished KCl induced contractile forces to 71% (p = 0.002) and NE induced contractions to 80% (p = 0.037), in contrast to HEPES-supplemented Krebs-Henseleit solution (HKH) and TiProtec solution. KCl-normalized NE contractions were not affected by storage. NE EC50 values were slightly lower (7.1E-8 vs. 7.5E-8, p = 0.019) after storage in HKH, with no changes after storage in the other solutions. Endothelium-dependent responses to ACh were not affected by storage. ET-1 induced contractions were attenuated after storage in HDMEM (77%, p = 0.002), HKH (75%, p = 0.020), and TiProtec (73%, p = 0.010) with no changes in normalized constrictions. ET-1 EC50 values were not affected by storage. Conclusion: Loss of contractility after storage in HDMEM may reflect the lower content of dextrose. There was no specific attenuation of adrenoceptor, ET-receptor, or ACh receptor mediated signal transduction after storage in any of the media. HKH or TiProtec are equally suitable cold storage solutions for ex vivo measurements. (© 2022 S. Karger AG, Basel.)
Databáze:	MEDLINE
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