| Autor: |
Satokar VV; Liggins Institute, University of Auckland, Auckland, New Zealand., Vickers MH; Liggins Institute, University of Auckland, Auckland, New Zealand., Reynolds CM; Liggins Institute, University of Auckland, Auckland, New Zealand.; University College Dublin Conway Institute of Biomolecular and Biomedical Research, Dublin, Ireland., Ponnampalam AP; Department of Physiology, University of Auckland, Auckland, New Zealand.; Department of Obstetrics and Gynaecology, University of Auckland, Auckland, New Zealand., Firth EC; Liggins Institute, University of Auckland, Auckland, New Zealand., Garg ML; Nutraceuticals Research Program, School of Biomedical Sciences and Pharmacy, University of Newcastle, Newcastle, New South Wales, Australia., Bridge-Comer PE; Liggins Institute, University of Auckland, Auckland, New Zealand., Cutfield WS; Liggins Institute, University of Auckland, Auckland, New Zealand.; A Better Start-National Science Challenge, University of Auckland, Auckland, New Zealand., Albert BB; Liggins Institute, University of Auckland, Auckland, New Zealand.; A Better Start-National Science Challenge, University of Auckland, Auckland, New Zealand. |
| Abstrakt: |
Fish oil (FO) supplements are consumed during pregnancy to increase dietary omega-3. However, FO is often oxidized past recommended limits. In rats, a large dose of highly oxidized FO substantially increased newborn mortality, but the effects of human-relevant doses of less oxidized oil are unknown. A dose-response study in rats was conducted to estimate the safe level of oxidation during pregnancy. Sprague-Dawley rat dams were mated, then individually housed and provided with a gel treatment on each day of pregnancy. Treatment groups differed only in the FO content of the gel; control (no oil), PV5, PV10, and PV40 [0.05 mL of FO oxidized to a peroxide value (PV) of 5, 10, or 40 meq/kg], or PV40(1 mL) (1 mL of PV40). A subset of dams was culled on gestational day 20 to enable sampling, and the remainder were allowed to give birth. Newborn mortality was recorded. Offspring were sampled on postnatal days 2 and 21 , and dams on day 21 . There were no signs of unwellness during pregnancy. However, there was markedly increased neonatal mortality affecting the PV40(1 mL) (12.8%) and PV40 (6.3%) groups, but not the control, PV5, or PV10 groups (1%-1.4%). Dietary-oxidized FO altered the expression of placental genes involved in antioxidant pathways and the production of free radicals. Highly oxidized FO was toxic in rat pregnancy leading to a marked increase in mortality even at a human-relevant dose. We observed no toxic effects of FOs with PV ≤10 meq/kg, suggesting that this is an appropriate maximum limit. |
