Establishment of well-differentiated camelid airway cultures to study Middle East respiratory syndrome coronavirus.

Autor: Gultom M; Institute of Virology and Immunology (IVI), Bern and Mittelhäusern, Switzerland.; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.; Institute for Infectious Diseases, University of Bern, Bern, Switzerland., Kratzel A; Institute of Virology and Immunology (IVI), Bern and Mittelhäusern, Switzerland.; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland., Portmann J; Institute of Virology and Immunology (IVI), Bern and Mittelhäusern, Switzerland.; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland., Stalder H; Institute of Virology and Immunology (IVI), Bern and Mittelhäusern, Switzerland.; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland., Chanfon Bätzner A; Institute of Animal Pathology (COMPATH), Vetsuisse Faculty, University of Bern, Bern, Switzerland., Gantenbein H; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.; Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, Bern, Switzerland., Gurtner C; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.; Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, Bern, Switzerland., Ebert N; Institute of Virology and Immunology (IVI), Bern and Mittelhäusern, Switzerland.; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland., Gad HH; Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark., Hartmann R; Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark., Posthaus H; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.; Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, Bern, Switzerland., Zanolari P; Department of Clinical Veterinary Medicine, Ruminant Clinic, University of Bern, Bern, Switzerland., Pfaender S; Institute of Virology and Immunology (IVI), Bern and Mittelhäusern, Switzerland.; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland.; Department for Molecular & Medical Virology, Ruhr University Bochum, Bochum, Germany., Thiel V; Institute of Virology and Immunology (IVI), Bern and Mittelhäusern, Switzerland. volker.thiel@vetsuisse.unibe.ch.; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland. volker.thiel@vetsuisse.unibe.ch.; Multidisciplinary Center for Infectious Diseases, University of Bern, Bern, Switzerland. volker.thiel@vetsuisse.unibe.ch., Dijkman R; Institute of Virology and Immunology (IVI), Bern and Mittelhäusern, Switzerland. ronald.dijkman@unibe.ch.; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland. ronald.dijkman@unibe.ch.; Institute for Infectious Diseases, University of Bern, Bern, Switzerland. ronald.dijkman@unibe.ch.; Multidisciplinary Center for Infectious Diseases, University of Bern, Bern, Switzerland. ronald.dijkman@unibe.ch.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Jun 20; Vol. 12 (1), pp. 10340. Date of Electronic Publication: 2022 Jun 20.
DOI: 10.1038/s41598-022-13777-y
Abstrakt: In 2012, Middle East respiratory syndrome coronavirus (MERS-CoV) emerged in Saudi Arabia and was mostly associated with severe respiratory illness in humans. Dromedary camels are the zoonotic reservoir for MERS-CoV. To investigate the biology of MERS-CoV in camelids, we developed a well-differentiated airway epithelial cell (AEC) culture model for Llama glama and Camelus bactrianus. Histological characterization revealed progressive epithelial cellular differentiation with well-resemblance to autologous ex vivo tissues. We demonstrate that MERS-CoV displays a divergent cell tropism and replication kinetics profile in both AEC models. Furthermore, we observed that in the camelid AEC models MERS-CoV replication can be inhibited by both type I and III interferons (IFNs). In conclusion, we successfully established camelid AEC cultures that recapitulate the in vivo airway epithelium and reflect MERS-CoV infection in vivo. In combination with human AEC cultures, this system allows detailed characterization of the molecular basis of MERS-CoV cross-species transmission in respiratory epithelium.
(© 2022. The Author(s).)
Databáze: MEDLINE
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