| Autor: |
Gupta R; Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843-3122, USA., Rhee KY; Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843-3122, USA., Beagle SD; Department of Biology, Washington University in St. Louis, St. Louis, Missouri, 63130, USA., Chawla R; Department of Integrative Structural and Computational Biology, Scripps Research, La Jolla, CA 92037, USA., Perdomo N; Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843-3122, USA., Lockless SW; Department of Biology, Texas A&M University, College Station, TX 77843-3258, USA., Lele PP; Artie McFerrin Department of Chemical Engineering, Texas A&M University, College Station, TX 77843-3122, USA. |
| Abstrakt: |
Indole is a major component of the bacterial exometabolome, and the mechanisms for its wide-ranging effects on bacterial physiology are biomedically significant, although they remain poorly understood. Here, we determined how indole modulates the functions of a widely conserved motility apparatus, the bacterial flagellum. Our experiments in Escherichia coli revealed that indole influences the rotation rates and reversals in the flagellum's direction of rotation via multiple mechanisms. At concentrations higher than 1 mM, indole decreased the membrane potential to dissipate the power available for the rotation of the motor that operates the flagellum. Below 1 mM, indole did not dissipate the membrane potential. Instead, experiments and modeling indicated that indole weakens cooperative protein interactions within the flagellar complexes to inhibit motility. The metabolite also induced reversals in the rotational direction of the motor to promote a weak chemotactic response, even when the chemotaxis response regulator, CheY, was lacking. Experiments further revealed that indole does not require the transporter Mtr to cross the membrane and influence motor functions. Based on these findings, we propose that indole modulates intra- and inter-protein interactions in the cell to influence several physiological functions. |
