Inducing and measuring apoptotic cell death in mouse pancreatic β-cells and in isolated islets.
| Autor: | McKimpson WM; Department of Medicine (Endocrinology), Columbia University, New York, NY 10032, USA., Kitsis RN; Departments of Medicine and Cell Biology, Wilf Family Cardiovascular Research Institute, Einstein-Mount Sinai Diabetes Research Center, and Albert Einstein Cancer Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2022 Apr 11; Vol. 3 (2), pp. 101287. Date of Electronic Publication: 2022 Apr 11 (Print Publication: 2022). |
| DOI: | 10.1016/j.xpro.2022.101287 |
| Abstrakt: | Type 2 diabetes is mediated by insulin resistance and pancreatic β-cell failure, the latter reflecting a combination of β-cell dysfunction, dedifferentiation, and apoptosis. Quantification of β-cell apoptosis in diabetes can be challenging both with respect to methodology and selection of clinically relevant inducers and readouts. This protocol describes approaches to measure cell death in immortalized β-cells, primary mouse islet preparations, and pancreatic tissue. The resulting information may be useful for mechanistic studies and assessment of the contribution of β-cell death to pathogenesis. For complete details on the use and execution of this protocol, please refer to McKimpson et al. (2021). Competing Interests: The authors declare no competing interests. (© 2022 The Author(s).) |
| Databáze: | MEDLINE |
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