In vitro study on efficacy of PHELA, an African traditional drug against SARS-CoV-2.

Autor: Matsabisa MG; Department of Pharmacology, School of Medicine, Faculty of Health Sciences, University of the Free State, Bloemfontein, 9300, South Africa. MatsabisaMG@ufs.ac.za., Alexandre K; Synthetic Biology Centre, Next Generation Health Cluster, Council for Scientific and Industrial Research, Pretoria, Gauteng, South Africa. AAlexandre@csir.co.za., Ibeji CU; Department of Pure and Industrial Chemistry, Faculty of Physical Sciences, University of Nigeria, Nsukka, 410001, Nigeria., Tripathy S; Department of Pharmacology, School of Medicine, Faculty of Health Sciences, University of the Free State, Bloemfontein, 9300, South Africa., Erukainure OL; Department of Pharmacology, School of Medicine, Faculty of Health Sciences, University of the Free State, Bloemfontein, 9300, South Africa., Malatji K; Synthetic Biology Centre, Next Generation Health Cluster, Council for Scientific and Industrial Research, Pretoria, Gauteng, South Africa., Chauke S; Synthetic Biology Centre, Next Generation Health Cluster, Council for Scientific and Industrial Research, Pretoria, Gauteng, South Africa., Okole B; Advanced Agriculture and Food Cluster, Council for Scientific and Industrial Research, Pretoria, South Africa., Chabalala HP; Department of Science and Innovation, Indigenous Knowledge-based Technology Innovations, Brummeria, Pretoria, 0001, South Africa.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Jun 19; Vol. 12 (1), pp. 10305. Date of Electronic Publication: 2022 Jun 19.
DOI: 10.1038/s41598-022-13599-y
Abstrakt: In 2019, coronavirus has made the third apparition in the form of SARS-CoV-2, a novel strain of coronavirus that is extremely pathogenic and it uses the same receptor as SARS-CoV, the angiotensin-converting enzyme 2 (ACE2). However, more than 182 vaccine candidates have been announced; and 12 vaccines have been approved for use, although, even vaccinated individuals are still vulnerable to infection. In this study, we investigated PHELA, recognized as an herbal combination of four exotic African medicinal plants namely; Clerodendrum glabrum E. Mey. Lamiaceae, Gladiolus dalenii van Geel, Rotheca myricoides (Hochst.) Steane & Mabb, and Senna occidentalis (L.) Link; as a candidate therapy for COVID-19. In vitro testing found that PHELA inhibited > 90% of SARS-CoV-2 and SARS-CoV infection at concentration levels of 0.005 mg/ml to 0.03 mg/ml and close to 100% of MERS-CoV infection at 0.1 mg/ml to 0.6 mg/ml. The in vitro average IC 50 of PHELA on SARS-COV-2, SARS-CoV and MERS-COV were ~ 0.01 mg/ml. Secondly in silico docking studies of compounds identified in PHELA showed very strong binding energy interactions with the SARS-COV-2 proteins. Compound 5 showed the highest affinity for SARS-COV-2 protein compared to other compounds with the binding energy of - 6.8 kcal mol -1 . Our data showed that PHELA has potential and could be developed as a COVID-19 therapeutic.
(© 2022. The Author(s).)
Databáze: MEDLINE
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