Toxicity of palytoxin, purified ovatoxin-a, ovatoxin-d and extracts of Ostreopsis cf. ovata on the Caco-2 intestinal barrier model.

Autor: Gémin MP; IFREMER, Phycotoxins Laboratory, F-44311 Nantes, France., Lanceleur R; ANSES, Fougères Laboratory, Toxicology of Contaminants Unit, French Agency for Food, Environmental and Occupational Health & Safety, Fougères 35306, France., Meslier L; ANSES, Fougères Laboratory, Toxicology of Contaminants Unit, French Agency for Food, Environmental and Occupational Health & Safety, Fougères 35306, France., Hervé F; IFREMER, Phycotoxins Laboratory, F-44311 Nantes, France., Réveillon D; IFREMER, Phycotoxins Laboratory, F-44311 Nantes, France., Amzil Z; IFREMER, Phycotoxins Laboratory, F-44311 Nantes, France., Ternon E; Sorbonne Université, CNRS, Laboratoire d'Océanographie de Villefranche, UMR 709, BP 28, F-06230 Villefranche-sur-Mer, France., Thomas OP; Marine Biodiscovery, School of Chemistry and Ryan Institute, National University of Ireland Galway, University Road, H91TK33 Galway, Ireland., Fessard V; ANSES, Fougères Laboratory, Toxicology of Contaminants Unit, French Agency for Food, Environmental and Occupational Health & Safety, Fougères 35306, France. Electronic address: valerie.fessard@anses.fr.
Jazyk: angličtina
Zdroj: Environmental toxicology and pharmacology [Environ Toxicol Pharmacol] 2022 Aug; Vol. 94, pp. 103909. Date of Electronic Publication: 2022 Jun 17.
DOI: 10.1016/j.etap.2022.103909
Abstrakt: Human intoxications in the Mediterranean Sea have been linked to blooms of the dinoflagellate Ostreopsis cf. ovata, producer of palytoxin (PlTX)-like toxins called ovatoxins (OVTXs). Exposure routes include only inhalation and contact, although PlTX-poisoning by seafood has been described in tropical regions. To address the impact of OVTXs on the intestinal barrier, dinoflagellate extracts, purified OVTX-a and -d and PlTX were tested on differentiated Caco-2 cells. Viability, inflammatory response and barrier integrity were recorded after 24 h treatment. OVTX-a and -d were not cytotoxic up to 20 ng/mL but increased IL-8 release, although to a lesser extent compared to PlTX. While PlTX and OVTX-a (at 0.5 and 5 ng/mL respectively) affected intestinal barrier integrity, OVTX-d up to 5 ng/mL did not. Overall, OVTX-d was shown to be less toxic than OVTX-a and PlTX. Therefore, oral exposure to OVTX-a and -d could provoked lower acute toxicity than PlTX.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: