The exposure in ovo of embryos belonging to Amazonian turtle species Podocnemis expansa (Testudines) to commercial glyphosate and fipronil formulations impairs their growth and changes their skeletal development.

Autor: Mendonça JDS; Programa de Pós-Graduação em Ecologia, Evolução e Biodiversidade, Instituto de Biociências, Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Rio Claro, SP, Brazil. Electronic address: ju_smendonca@yahoo.com.br., Hirano LQL; Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília (UNB), Brasília, DF, Brazil., Santos ALQ; Laboratório de Ensino e Pesquisa em Animais Silvestres, Universidade Federal de Uberlândia (UFU), Uberlândia, MG, Brazil., de Melo E Silva D; Laboratório de Genética e Mutagênese, Instituto de Ciências Biológicas, Universidade Federal de Goiás (UFG), Goiânia, GO, Brazil., de Castro Pereira C; Laboratório Multidisciplinar em Morfologia e Ontogenia, Instituto de Ciências Biológicas, Universidade Federal de Goiás (UFG), Goiânia, GO, Brazil., Costa MS; Laboratório Multidisciplinar em Morfologia e Ontogenia, Instituto de Ciências Biológicas, Universidade Federal de Goiás (UFG), Goiânia, GO, Brazil., de Lima PN; Laboratório Multidisciplinar em Morfologia e Ontogenia, Instituto de Ciências Biológicas, Universidade Federal de Goiás (UFG), Goiânia, GO, Brazil., Araújo APDC; Laboratório de Toxicologia Amplicada ao Meio Ambiente, Programa de Pós-Graduação em Conservação dos Recursos Naturais do Cerrado, Instituto Federal Goiano - Campus Urutaí, GO, Brazil; Programa de Pós-Graduação em Ciências Ambientais, Universidade Federal de Goiás (UFG), Goiânia, GO, Brazil., Malafaia G; Laboratório de Toxicologia Amplicada ao Meio Ambiente, Programa de Pós-Graduação em Conservação dos Recursos Naturais do Cerrado, Instituto Federal Goiano - Campus Urutaí, GO, Brazil; Programa de Pós-Graduação em Biotecnologia e Biodiversidade, Instituição Federal Goiano e Universidade Federal de Goiás, GO, Brazil; Programa de Pós-Graduação em Ecologia, Conservação e Biodiversidade, Universidade Federal de Uberlândia (UFU), Uberlândia, MG, Brazil., Ambrósio IS; Instituto de Ciências Exatas e Naturais do Pontal, Universidade Federal de Uberlândia (UFU), Ituiutaba, MG, Brazil., de Moura Bife Castilho L; Instituto de Ciências Exatas e Naturais do Pontal, Universidade Federal de Uberlândia (UFU), Ituiutaba, MG, Brazil., Dos Santos ALR; Instituto de Ciências Exatas e Naturais do Pontal, Universidade Federal de Uberlândia (UFU), Ituiutaba, MG, Brazil., de Faria AM; Instituto de Ciências Exatas e Naturais do Pontal, Universidade Federal de Uberlândia (UFU), Ituiutaba, MG, Brazil., de Andrade DV; Departamento de Biodiversidade, Instituto de Biociências, Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), Rio Claro, SP, Brazil., Vieira LG; Laboratório Multidisciplinar em Morfologia e Ontogenia, Instituto de Ciências Biológicas, Universidade Federal de Goiás (UFG), Goiânia, GO, Brazil.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2022 Oct 10; Vol. 842, pp. 156709. Date of Electronic Publication: 2022 Jun 17.
DOI: 10.1016/j.scitotenv.2022.156709
Abstrakt: Pesticides are widely used in agricultural production; moreover, they can have direct and indirect effect on both flora and fauna. Aquatic organisms, among other animals, including reptiles, are mainly susceptible to contamination effects. Accordingly, the aim of the present study is to test the hypothesis that the incubation of Podocnemis expansa eggs in substrate added with glyphosate and fipronil formulations changes their viability, interferes with their growth and induces bone alterations. Eggs collected in natural environment were artificially incubated in sand moistened with water added with glyphosate Atar 48, at concentrations of 65 or 6500 μg/L (groups G1 and G2, respectively), and with fipronil Regent 800 WG at 4 or 400 μg/L (groups F1 and F2, respectively) or, yet, with the combination of 65 μg/L glyphosate and 4 μg/L fipronil, or with 6500 μg/L glyphosate and 400 μg/L fipronil (groups GF1 and GF2, respectively). The level of exposure to the herein assessed pesticides was quantified at the end of the incubation period; it was done by dosing its concentration in eggshells. Eggs exposed to the tested pesticides did not have their viability affected by it; however, all embryos exposed to the tested pesticides showed lowered body mass at hatch, as well as impaired development. In addition, bone malformation in the scleral ossicular ring was observed in individuals in groups F1, F2 and GF1. Pesticides accumulated in eggshells at concentrations related to exposure level. Thus, the recorded results have evidenced some remarkably relevant, and previously unknown, impacts associated with the exposure of a species listed as lower risk/conservation dependent, which spends most of its life in the water, to two widely used pesticides, at a very sensitive stage of its life, namely: egg incubation on land.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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