Bone regeneration in critical-sized mandibular symphysis defects using bioceramics with or without bone marrow mesenchymal stem cells in healthy, diabetic, osteoporotic, and diabetic-osteoporotic rats.

Autor: Camacho-Alonso F; Department of Oral Surgery, University of Murcia, Murcia, Spain. Electronic address: fcamacho@um.es., Tudela-Mulero MR; Department of Oral Surgery, University of Murcia, Murcia, Spain., Buendía AJ; Department of Histology and Pathological Anatomy, University of Murcia, Murcia, Spain., Navarro JA; Department of Histology and Pathological Anatomy, University of Murcia, Murcia, Spain., Pérez-Sayáns M; Department of Oral Medicine, Oral Surgery and Implantology, University of Santiago de Compostela, Spain. MedOralRes Group, Health Research Institute of Santiago de Compostela (IDIS). Santiago de Compostela, Spain., Mercado-Díaz AM; In private dental practice, Murcia, Spain.
Jazyk: angličtina
Zdroj: Dental materials : official publication of the Academy of Dental Materials [Dent Mater] 2022 Aug; Vol. 38 (8), pp. 1283-1300. Date of Electronic Publication: 2022 Jun 16.
DOI: 10.1016/j.dental.2022.06.019
Abstrakt: Objectives: To compare new bone formation in mandibular critical-sized bone defects (CSBDs) in healthy, diabetic, osteoporotic, and diabetic-osteoporotic rats filled with bioceramics (BCs) with or without bone marrow mesenchymal stem cells (BMSCs).
Methods: A total of 64 adult female Sprague-Dawley rats were randomized into four groups (n = 16 per group): Group 1 healthy, Group 2 diabetic, Group 3 osteoporotic, and Group 4 diabetic-osteoporotic rats. Streptozotocin was used to induce type 1 diabetes in Group 2 and 4, while bilateral ovariectomy was used to induce osteoporosis in Group 3 and 4. The central portion of the rat mandibular symphysis was used as a physiological CSBD. In each group, eight defects were filled with BC (hydroxypatatite 60% and β-tricalcium phosphate 40%) alone and eight with BMSCs cultured on BC. The animals were sacrificed at 4 and 8 weeks, and the mandibles were processed for micro-computed tomography to analyze radiological union and bone mineral density (BMD); histological analysis of the bone union; and immunohistochemical analysis, which included immunoreactivity of vascular endothelial growth factor (VEGF) and bone morphogenetic protein 2 (BMP-2).
Results: In all groups (healthy, diabetics, osteoporotics, and diabetics-osteoporotics), the CSBDs filled with BC + BMSCs showed greater radiological bone union, BMD, histological bone union, and more VEGF and BMP-2 positivity, in comparison with CSBDs treated with BC alone (at 4 and 8 weeks).
Conclusions: Application of BMSCs cultured on BCs improves bone regeneration in CSBDs compared with application of BCs alone in healthy, diabetic, osteoporotic, and diabetic-osteoporotic rats.
(Copyright © 2022. Published by Elsevier Inc.)
Databáze: MEDLINE
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