Genome-wide association mapping of resistance to the sorghum aphid in Sorghum bicolor.

Autor: Punnuri SM; Agriculture Research Station, 1005 State University Dr, Fort Valley State University, Fort Valley, GA 31030, United States of America. Electronic address: punnuris@fvsu.edu., Ayele AG; Agriculture Research Station, 1005 State University Dr, Fort Valley State University, Fort Valley, GA 31030, United States of America., Harris-Shultz KR; USDA-ARS, Crop Genetics and Breeding Research Unit, 115 Coastal Way, Tifton, GA 31793, United States of America., Knoll JE; USDA-ARS, Crop Genetics and Breeding Research Unit, 115 Coastal Way, Tifton, GA 31793, United States of America., Coffin AW; USDA-ARS, Southeast Watershed Research Laboratory, 2316 Rainwater Road, Tifton, GA 31793, United States of America., Tadesse HK; USDA-ARS, Southeast Watershed Research Laboratory, 2316 Rainwater Road, Tifton, GA 31793, United States of America., Armstrong JS; USDA-ARS, Wheat Peanut and Other Field Crops Research Unit, 1301 N. Western Road, Stillwater, OK 74075, United States of America., Wiggins TK; Agriculture Research Station, 1005 State University Dr, Fort Valley State University, Fort Valley, GA 31030, United States of America., Li H; University of Georgia, Institute of Bioinformatics, 120 Green Street, Athens, GA 30602, United States of America., Sattler S; USDA-ARS, Wheat, Sorghum and Forage Research Unit, Lincoln, NE 68583, United States of America., Wallace JG; University of Georgia, Department of Crop & Soil Sciences, 120 Carlton Street, Athens, GA 30602, United States of America.
Jazyk: angličtina
Zdroj: Genomics [Genomics] 2022 Jul; Vol. 114 (4), pp. 110408. Date of Electronic Publication: 2022 Jun 15.
DOI: 10.1016/j.ygeno.2022.110408
Abstrakt: Since 2013, the sorghum aphid (SA), Melanaphis sorghi (Theobald), has been a serious pest that hampers all types of sorghum production in the U.S. Known sorghum aphid resistance in sorghum is limited to a few genetic regions on SBI-06. In this study, a subset of the Sorghum Association Panel (SAP) was used along with some additional lines to identify genomic regions that confer sorghum aphid resistance. SAP lines were grown in the field and visually evaluated for SA resistance during the growing seasons of 2019 and 2020 in Tifton, GA. In 2020, the SAP accessions were also evaluated for SA resistance in the field using drone-based high throughput phenotyping (HTP). Flowering time was recorded in the field to confirm that our methods were sufficient for identifying known quantitative trait loci (QTL). This study combined phenotypic data from field-based visual ratings and reflectance data to identify genome-wide associated (GWAS) marker-trait associations (MTA) using genotyping-by-sequencing (GBS) data. Several MTAs were identified for SA-related traits across the genome, with a few common markers that were consistently identified on SBI-08 and SBI-10 for aphid count and plant damage, as well as loci for reflectance-based traits on SBI-02, SBI-03, and SBI-05. Candidate genes encoding leucine-rich repeats (LRR), Avr proteins, lipoxygenases (LOXs), calmodulins (CAM) dependent protein kinase, WRKY transcription factors, flavonoid biosynthesis genes, and 12-oxo-phytodienoic acid reductase were identified near SNPs that had significant associations with different SA traits. In this study, flowering time-related genes were also identified as a positive control for the methods. The total phenotypic variation explained by significant SNPs across SA-scored traits, reflectance data, and flowering time ranged from 6 to 61%, while the heritability value ranged from 4 to 69%. This study identified three new sources of resistant lines to sorghum aphid. These results supported the existing literature, and also revealed several new loci. Markers identified in this study will support marker-assisted breeding for sorghum aphid resistance.
(Published by Elsevier Inc.)
Databáze: MEDLINE
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