Post-thaw dilution of Rhamdia quelen sperm improves the reproductive success.

Autor: França TS; Aquam Research Group, Animal Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91540-000, Brazil. Electronic address: thalesfranca@gmail.com., Gomes IC; Aquam Research Group, Animal Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91540-000, Brazil. Electronic address: itamar_cossina@yahoo.com.br., Sanches EA; Fishery Engineering Course and Aquaculture Centre (CAUNESP) São Paulo State University, Registro, São Paulo 11900-000, Brazil. Electronic address: eduardo.sanches@unesp.br., Atehortúa MP; Department of Agricultural and Aquacultural Sciences, Faculty of Natural Resources, Catholic University of Temuco, Temuco 4781312, Chile. Electronic address: waira122@gmail.com., Teixeira NS; Aquam Research Group, Animal Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91540-000, Brazil. Electronic address: nati_st@hotmail.com., Rodrigues RB; Aquam Research Group, Animal Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91540-000, Brazil. Electronic address: rrodrigues1903@gmail.com., de Freitas TR; Aquam Research Group, Animal Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91540-000, Brazil. Electronic address: thaiza.rfreitas@gmail.com., Galuppo AG; Aquam Research Group, Animal Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91540-000, Brazil. Electronic address: andreagalpo@yahoo.com.br., Quirino M; Dept. Veterinary Medicine, Federal University of Rio Grande do Sul, UFRGS, Porto Alegre, Rio Grande do Sul 91540-000, Brazil. Electronic address: monikequirino@gmail.com., Benato JL; Aquam Research Group, Animal Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91540-000, Brazil. Electronic address: jhonybenato@hotmail.com., Machado TLF; Aquam Research Group, Animal Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91540-000, Brazil. Electronic address: thalesflores@hotmail.com., Marques LS; Aquam Research Group, Animal Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91540-000, Brazil. Electronic address: lismarx@gmail.com., Bustamante-Filho IC; Biotechnology Laboratory, University of Vale of Taquari - Univates, Avelio Tallini Street, 171, Lajeado, Rio Grande do Sul, Brazil. Electronic address: ivanbustamante@univates.br., Bortolozzo FP; Dept. Veterinary Medicine, Federal University of Rio Grande do Sul, UFRGS, Porto Alegre, Rio Grande do Sul 91540-000, Brazil. Electronic address: fpbortol@ufrgs.br., Streit Jr DP Jr; Aquam Research Group, Animal Science Research Program, Federal University of Rio Grande do Sul, Porto Alegre, Rio Grande do Sul 91540-000, Brazil. Electronic address: danilo.streit@ufrgs.br.
Jazyk: angličtina
Zdroj: Animal reproduction science [Anim Reprod Sci] 2022 Aug; Vol. 243, pp. 107018. Date of Electronic Publication: 2022 Jun 11.
DOI: 10.1016/j.anireprosci.2022.107018
Abstrakt: The aim was to evaluate the effect of a post-thaw dilution of Rhamdia quelen sperm in 1.1% NaCl (325 mOsm kg -1 ; pH 7.6; 24 °C) solution on the quality and reproductive capacity. Sperm from eight males were cryopreservation in nitrogen vapor at - 170 °C for 18 h in 0.25 mL straws in a freezing medium containing 5% fructose, 5% Powdered milk, and 10% methanol. The samples were thawed and post-thaw diluted (1:20) in NaCl solution or not (control). The higher spermatozoa velocities were observed in the post-thaw diluted samples (curvilinear (VCL) - 69 ± 11 µm s -1 ; average path (VAP) - 45 ± 8 µm s -1 ; straight-line (VSL) - 43 ± 8 µm s -1 ) compared to the control (VCL - 47 ± 10 µm s -1 ; VAP - 31 ± 6 µm s -1 ; VSL - 30 ± 6 µm s -1 ). Greater straightness (STR), progression (PROG), and beat cross frequency (BCF) were observed in the post-thaw diluted samples (STR - 96 ± 7%; PROG - 666 ± 128 µm; BCF - 42 ± 2 Hz) than in control (STR - 95 ± 5%; PROG - 463 ± 92 µm; BCF - 40 ± 2 Hz). The strongly curled tail was the only morphology change that differ between the post-thaw diluted (5 ± 2%) and control (2 ± 1%). Membrane integrity, mitochondrial activity, and normal larvae rate were not different between treatments. Fertilization and hatching were higher in the post-thaw diluted sperm (93 ± 3%; 82 ± 9%) when compared to control samples (65 ± 13%; 55 ± 17%). Were used oocytes from one female, limiting these results. The post-thaw dilution improved the sperm kinetics and reproductive parameters. Thus, this methodology can be included in the sperm cryopreservation protocol for R. quelen.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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