Characterization of triatomine bloodmeal sources using direct Sanger sequencing and amplicon deep sequencing methods.

Autor: Balasubramanian S; Department of Veterinary Integrative Biosciences, MS 4458, Texas A&M University, College Station, TX, 77843, USA., Curtis-Robles R; Department of Veterinary Integrative Biosciences, MS 4458, Texas A&M University, College Station, TX, 77843, USA., Chirra B; Department of Epidemiology and Biostatistics, Texas A&M Health Science Center, College Station, TX, 77843, USA.; Department of Microbial Pathogenesis & Immunology, Texas A&M University Health Science Center, College Station, TX, 77843, USA., Auckland LD; Department of Veterinary Integrative Biosciences, MS 4458, Texas A&M University, College Station, TX, 77843, USA., Mai A; Department of Epidemiology and Biostatistics, Texas A&M Health Science Center, College Station, TX, 77843, USA.; Florida Department of Health, Public Health Research Unit, Tallahassee, FL, 32399, USA., Bocanegra-Garcia V; Centro de Biotecnología Genómica, Instituto Politécnico Nacional, Reynosa, TAMPS, Mexico., Clark P; Austin Zoo, Austin, TX, 78736, USA., Clark W; SNBL USA, Ltd., Alice, TX, 78332, USA., Cottingham M; SNBL USA, Ltd., Alice, TX, 78332, USA., Fleurie G; SNBL USA, Ltd., Alice, TX, 78332, USA.; Charles River Laboratories, Reno, NV, 89511, USA., Johnson CD; Genomics and Bioinformatics Services, Texas A&M Agrilife Research, College Station, TX, 77843, USA., Metz RP; Genomics and Bioinformatics Services, Texas A&M Agrilife Research, College Station, TX, 77843, USA., Wang S; Genomics and Bioinformatics Services, Texas A&M Agrilife Research, College Station, TX, 77843, USA., Hathaway NJ; Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA., Bailey JA; Department of Pathology and Laboratory Medicine, Brown University, Providence, RI, 02912, USA., Hamer GL; Department of Entomology, MS2475, Texas A&M University, College Station, TX, 77843, USA., Hamer SA; Department of Veterinary Integrative Biosciences, MS 4458, Texas A&M University, College Station, TX, 77843, USA. shamer@cvm.tamu.edu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Jun 17; Vol. 12 (1), pp. 10234. Date of Electronic Publication: 2022 Jun 17.
DOI: 10.1038/s41598-022-14208-8
Abstrakt: Knowledge of host associations of blood-feeding vectors may afford insights into managing disease systems and protecting public health. However, the ability of methods to distinguish bloodmeal sources varies widely. We used two methods-Sanger sequencing and amplicon deep sequencing-to target a 228 bp region of the vertebrate Cytochrome b gene and determine hosts fed upon by triatomines (n = 115) collected primarily in Texas, USA. Direct Sanger sequencing of PCR amplicons was successful for 36 samples (31%). Sanger sequencing revealed 15 distinct host species, which included humans, domestic animals (Canis lupus familiaris, Ovis aries, Gallus gallus, Bos taurus, Felis catus, and Capra hircus), wildlife (Rattus rattus, Incilius nebulifer, Sciurus carolinensis, Sciurus niger, and Odocoileus virginianus), and captive animals (Panthera tigris, Colobus spp., and Chelonoidis carbonaria). Samples sequenced by the Sanger method were also subjected to Illumina MiSeq amplicon deep sequencing. The amplicon deep sequencing results (average of 302,080 usable reads per sample) replicated the host community revealed using Sanger sequencing, and detected additional hosts in five triatomines (13.9%), including two additional blood sources (Procyon lotor and Bassariscus astutus). Up to four bloodmeal sources were detected in a single triatomine (I. nebulifer, Homo sapiens, C. lupus familiaris, and S. carolinensis). Enhanced understanding of vector-host-parasite networks may allow for integrated vector management programs focusing on highly-utilized and highly-infected host species.
(© 2022. The Author(s).)
Databáze: MEDLINE
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