Multilocus sequence typing (MLST) analysis reveals many novel genotypes and a high level of genetic diversity in Candida tropicalis isolates from Italy and Africa.

Autor: Dougue AN; Antimicrobial & Biocontrol Agents Unit (AmBcAU), Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon., El-Kholy MA; Department of Microbiology and Biotechnology, Division of Clinical and Biological Sciences, College of Pharmacy, Arab Academy for Science, Technology and Maritime Transport (AASTMT), Alexandria, Egypt., Giuffrè L; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy., Galeano G; Department of Veterinary Sciences, University of Messina, Messina, Italy., D Aleo F; Laboratory of Clinical Microbiology, Great Metropolitan Hospital of Reggio Calabria, Reggio Calabria, Italy., Kountchou CL; Research Unit of Laboratory of Microbiology and Antimicrobial Substances (RUMAS), Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon.; Institute of Medical Research and Medicinal Plant Studies, Center for Medical Research in Health and Priority Pathologies, Yaoundé, Cameroon., Nangwat C; Research Unit of Laboratory of Microbiology and Antimicrobial Substances (RUMAS), Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon., Dzoyem JP; Research Unit of Laboratory of Microbiology and Antimicrobial Substances (RUMAS), Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon., Giosa D; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy., Pernice I; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy., Shawky SM; Department of Microbiology, Medical Research Institute, University of Alexandria, Alexandria, Egypt., Ngouana TK; Antimicrobial & Biocontrol Agents Unit (AmBcAU), Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon.; Biomedical Research Unit, Laboratoire Sion, Yaoundé, Cameroon., Boyom FF; Antimicrobial & Biocontrol Agents Unit (AmBcAU), Laboratory for Phytobiochemistry and Medicinal Plants Studies, Department of Biochemistry, Faculty of Science, University of Yaoundé I, Yaoundé, Cameroon., Romeo O; Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.
Jazyk: angličtina
Zdroj: Mycoses [Mycoses] 2022 Nov; Vol. 65 (11), pp. 989-1000. Date of Electronic Publication: 2022 Jul 07.
DOI: 10.1111/myc.13483
Abstrakt: Background: Candida tropicalis is a human pathogenic yeast frequently isolated in Latin America and Asian-Pacific regions, although recent studies showed that it is also becoming increasingly widespread throughout several African and south-European countries. Nevertheless, relatively little is known about its global patterns of genetic variation as most of existing multilocus sequence typing (MLST) data come from Asia and there are no genotyped African isolates.
Objectives: We report detailed genotyping data from a large set of C. tropicalis isolates recovered from different clinical sources in Italy, Egypt and Cameroon in order to expand the allele/genotype library of MLST database (https://pubmlst.org/ctropicalis), and to explore the genetic diversity in this species.
Methods: A total of 103 C. tropicalis isolates were genotyped using the MLST scheme developed for this species. All isolates were also tested for in vitro susceptibility to various antifungals to assess whether certain genotypes were associated with drug-resistance.
Results and Conclusions: A total of 104 different alleles were detected across the MLST-loci investigated. The allelic diversity found at these loci resulted in 51 unique MLST genotypes of which 36 (70.6%) were novel. Global optimal eBURST analysis identified 18 clonal complexes (CCs) and confirm the existence of a specific Italian-cluster (CC36). Three CCs were also statistically associated with fluconazole resistance, which was elevated in Cameroon and Egypt. Our data show high genetic diversity in our isolates suggesting that the global population structure of C. tropicalis is still poorly understood. Moreover, its clinical impact in Italy, Egypt and Cameroon appears to be relevant and should be carefully considered.
(© 2022 The Authors. Mycoses published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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