Controllable assembly of synthetic constructs with programmable ternary DNA interaction.

Autor:	Cui H; School of Life Sciences, Tsinghua University-Peking University Center for Life Sciences, Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China., Zhang T; School of Life Sciences, Tsinghua University-Peking University Center for Life Sciences, Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China., Kong Y; Institute of Chemical Biology and Nanomedicine; State Key Laboratory of Chemo/Biosensing and Chemometrics; Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology; College of Chemistry and Chemical Engineering; Hunan University, Changsha 410082, China., Xing H; Institute of Chemical Biology and Nanomedicine; State Key Laboratory of Chemo/Biosensing and Chemometrics; Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology; College of Chemistry and Chemical Engineering; Hunan University, Changsha 410082, China., Wei B; School of Life Sciences, Tsinghua University-Peking University Center for Life Sciences, Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China.
Jazyk:	angličtina
Zdroj:	Nucleic acids research [Nucleic Acids Res] 2022 Jul 08; Vol. 50 (12), pp. 7188-7196.
DOI:	10.1093/nar/gkac478
Abstrakt:	Compared with the dual binding components in a binary interaction, the third component of a ternary interaction often serves as modulator or regulator in biochemical processes. Here, we presented a programmable ternary interaction strategy based on the natural DNA triplex structure. With the DNA triplex-based ternary interaction, we have successfully demonstrated controllable hierarchical assemblies from nanometer scale synthetic DNA nanostructure units to micrometer scale live bacteria. A selective signaling system responsive to orthogonal nucleic acid signals via ternary interaction was also demonstrated. This assembly method could further enrich the diversified design schemes of DNA nanotechnology. (© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.)
Databáze:	MEDLINE
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