The central circadian regulator CCA1 functions in Glycine max during defense to a root pathogen, regulating the expression of genes acting in effector triggered immunity (ETI) and cell wall metabolism.

Autor: Niraula PM; Department of Biological Sciences, Mississippi State University, Mississippi State, MS, 39762, USA. Electronic address: pmniraula2@gmail.com., McNeece BT; Department of Biological Sciences, Mississippi State University, Mississippi State, MS, 39762, USA. Electronic address: brantmcneece13@yahoo.com., Sharma K; Department of Biological Sciences, Mississippi State University, Mississippi State, MS, 39762, USA. Electronic address: palpaleekeshav@yahoo.com., Alkharouf NW; Department of Computer and Information Sciences, Towson University, Towson, MD, 21252, USA. Electronic address: nalkharouf@towson.edu., Lawrence KS; Department of Entomology and Plant Pathology, Auburn University, 209 Life Science Building, Auburn, AL, 36849, USA. Electronic address: lawrekk@auburn.edu., Klink VP; Department of Biological Sciences, Mississippi State University, Mississippi State, MS, 39762, USA; USDA-ARS-NEA-BARC Molecular Plant Pathology Laboratory Building 004, Room 122, BARC-West, 10300 Baltimore Ave., Beltsville, MD, 20705, USA; Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS, 39762, USA; Center for Computational Sciences High Performance Computing Collaboratory, Mississippi State University, Mississippi State, MS, 39762, USA. Electronic address: Vincent.klink@USDA.gov.
Jazyk: angličtina
Zdroj: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2022 Aug 15; Vol. 185, pp. 198-220. Date of Electronic Publication: 2022 May 30.
DOI: 10.1016/j.plaphy.2022.05.028
Abstrakt: Expression of the central circadian oscillator components CIRCADIAN CLOCK ASSOCIATED 1 (CCA1), TIMING OF CAB1 (TOC1), GIGANTEA (GI), and CONSTANS (CO) occurs in Glycine max root cells (syncytia) parasitized by the nematode Heterodera glycines while undergoing resistance, indicating a defense role. GmCCA1-1 relative transcript abundance (RTA) in roots experiencing overexpression (OE) or RNA interference (RNAi) is characterized by rhythmic oscillations, compared to a ribosomal protein gene (GmRPS21) control. A GmCCA1-1 RTA change, advancing by 12 h, exists in H. glycines-infected as compared to uninfected controls in wild-type, H. glycines-resistant, G. max [Peking/PI 548402] . The G. max [Peking/PI 548402] transgenic controls exhibit the RTA change by 4 h post infection (hpi), not consistently occurring in the H. glycines-susceptible G. max [Williams 82/PI 518671] until 56 hpi. GmCCA1-1 expression is observed to be reduced in H. glycines-infected GmCCA1-1-OE roots as compared to non-infected transgenic roots with no significant change observed among RNAi roots. The GmCCA1-1 expression in transgenic GmCCA1-1-OE roots remains higher than control and RNAi roots. Decreased GmCCA1-1 mRNA among infected roots shows the altered expression is targeted by H. glycines. Gene expression of proven defense genes including 9 different mitogen activated protein kinases (GmMAPKs), NON-RACE SPECIFIC DISEASE RESISTANCE 1 (GmNDR1-1), RPM1-INTERACTING PROTEIN 4 (GmRIN4-4), and the secreted xyloglucan endotransglycosylase/hydrolase 43 (GmXTH43) in GmCCA1-1-OE and GmCCA1-1-RNAi roots, compared to controls, reveal a significant role of GmCCA1-1 expression in roots undergoing defense to H. glycines parasitism. The observation that GmCCA1-1 regulates GmXTH43 expression links the central circadian oscillator to the functionality of the secretion system.
(Copyright © 2022. Published by Elsevier Masson SAS.)
Databáze: MEDLINE
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