Human Immature Dental Pulp Stem Cells Did Not Graft into a Preexisting Human Lung Adenocarcinoma.

Autor: da Silva JM; Azidus Brazil, São Paulo, Brazil., Araldi RP; Genetics Laboratory, Butantan Institute, São Paulo, Brazil.; Cellavita Scientific Researches, São Paulo, Brazil.; Structural and Functional Biology Post-graduation Program, Federal University of São Paulo (UNIFESP), São Paulo, Brazil., Colozza-Gama GA; Structural and Functional Biology Post-graduation Program, Federal University of São Paulo (UNIFESP), São Paulo, Brazil., Pagani E; Azidus Brazil, São Paulo, Brazil., Sid A; Azidus Brazil, São Paulo, Brazil., Valverde CW; Cellavita Scientific Researches, São Paulo, Brazil., Kerkis I; Genetics Laboratory, Butantan Institute, São Paulo, Brazil.; Structural and Functional Biology Post-graduation Program, Federal University of São Paulo (UNIFESP), São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Case reports in oncology [Case Rep Oncol] 2022 Apr 21; Vol. 15 (1), pp. 413-422. Date of Electronic Publication: 2022 Apr 21 (Print Publication: 2022).
DOI: 10.1159/000523896
Abstrakt: Mesenchymal stem cell (MSC)-based therapies have been considered an attractive approach for treating Huntington's disease (HD). However, due to the pulmonary first-passage effect associated with intravenous infusion (the most commonly used route of MSC administration), there is a rising concern that the cells could be entrapped in the lungs and grafted (homing) into preexisting lung cancer. Herein, we report the case of a patient with HD enrolled in a cell therapy phase I clinical trial for HD treatment having a preexisting pulmonary nodule. The nodule was found at the trial screening. The patient was referred to a pulmonologist who considered the nodule non-cancer and authorized enrollment. The patient received four intravenous administrations of human immature dental pulp stem cells (hIDPSCs) at the dose of 1 × 10 6 cells/kg of body weight within 2 years. One month after the last dose, a computerized tomography scan showed nodule growth. A bronchoscopy biopsy showed primary lung adenocarcinoma. The neoplasm was surgically excised (lung superior right lobectomy). The patient is cured of the neoplasm. The tumor was sectioned into six fragments, which were subjected to RNA-seq. The transcriptome of each tumor section was compared with the transcriptome of infused hIDPSCs using two statistical approaches: principal component analysis and NOIseq. Both results demonstrated a linear distance between the hIDPSCs and the lung adenocarcinoma. These results suggest that the infused hIDPSCs neither home nor graft within the pulmonary nodule.
Competing Interests: The authors have no conflict of interest to declare.
(Copyright © 2022 by S. Karger AG, Basel.)
Databáze: MEDLINE
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