Microbial isolates with Anti-Pseudogymnoascus destructans activities from Western Canadian bat wings.

Autor: Forsythe A; Department of Biology, Faculty of Science, McMaster University, Hamilton, ON, L8S 4K1, Canada., Fontaine N; Department of Biological Sciences, Faculty of Science, Thompson Rivers University, Kamloops, BC, V2C 08C, Canada., Bissonnette J; Department of Biological Sciences, Faculty of Science, Thompson Rivers University, Kamloops, BC, V2C 08C, Canada., Hayashi B; Department of Biological Sciences, Faculty of Science, Thompson Rivers University, Kamloops, BC, V2C 08C, Canada., Insuk C; Department of Biology, Faculty of Science, McMaster University, Hamilton, ON, L8S 4K1, Canada.; Department of Biological Sciences, Faculty of Science, Thompson Rivers University, Kamloops, BC, V2C 08C, Canada., Ghosh S; Department of Biological Sciences, Faculty of Science, Thompson Rivers University, Kamloops, BC, V2C 08C, Canada.; Department of Genetics, Natural and Agricultural Sciences, University of the Free State, Bloemfontein, South Africa., Kam G; Department of Biological Sciences, Faculty of Science, Thompson Rivers University, Kamloops, BC, V2C 08C, Canada., Wong A; Department of Biological Sciences, Faculty of Science, Thompson Rivers University, Kamloops, BC, V2C 08C, Canada., Lausen C; Wildlife Conservation Society Canada, P.O. Box 606, Kaslo, BC, V0G 1M0, Canada. clausen@wcs.org., Xu J; Department of Biology, Faculty of Science, McMaster University, Hamilton, ON, L8S 4K1, Canada. jpxu@mcmaster.ca., Cheeptham N; Department of Biological Sciences, Faculty of Science, Thompson Rivers University, Kamloops, BC, V2C 08C, Canada. ncheeptham@tru.ca.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Jun 14; Vol. 12 (1), pp. 9895. Date of Electronic Publication: 2022 Jun 14.
DOI: 10.1038/s41598-022-14223-9
Abstrakt: Pseudogymnoascus destructans (Pd) is the causative agent of white-nose syndrome, which has resulted in the death of millions of bats in North America (NA) since 2006. Based on mortalities in eastern NA, the westward spread of infections likely poses a significant threat to western NA bats. To help prevent/reduce Pd infections in bats in western NA, we isolated bacteria from the wings of wild bats and screened for inhibitory activity against Pd. In total, we obtained 1,362 bacterial isolates from 265 wild bats of 13 species in western Canada. Among the 1,362 isolates, 96 showed inhibitory activity against Pd based on a coculture assay. The inhibitory activities varied widely among these isolates, ranging from slowing fungal growth to complete inhibition. Interestingly, host bats containing isolates with anti-Pd activities were widely distributed, with no apparent geographic or species-specific pattern. However, characteristics of roosting sites and host demography showed significant associations with the isolation of anti-Pd bacteria. Specifically, anthropogenic roosts and swabs from young males had higher frequencies of anti-Pd bacteria than those from natural roosts and those from other sex and age-groups, respectively. These anti-Pd bacteria could be potentially used to help mitigate the impact of WNS. Field trials using these as well as additional microbes from future screenings are needed in order to determine their effectiveness for the prevention and treatment against WNS.
(© 2022. The Author(s).)
Databáze: MEDLINE
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