| Autor: |
Iverson E; Department of Cell Biology & Molecular Genetics, Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland, USA., Griswold K; Department of Cell Biology & Molecular Genetics, Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland, USA., Song D; Fischell Department of Bioengineering, University of Maryland, College Park, Maryland, USA., Gagliardi TB; Department of Cell Biology & Molecular Genetics, Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland, USA., Hamidzadeh K; Department of Cell Biology & Molecular Genetics, Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland, USA., Kesimer M; Marsico Lung Institute, University of North Carolina, Chapel Hill, North Carolina, USA., Sinha S; Cancer Data Science Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, USA.; Center for Bioinformatics and Computational Biology, University of Maryland, College Park, Maryland, USA., Perry M; Department of Cell Biology & Molecular Genetics, Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland, USA., Duncan GA; Fischell Department of Bioengineering, University of Maryland, College Park, Maryland, USA., Scull MA; Department of Cell Biology & Molecular Genetics, Maryland Pathogen Research Institute, University of Maryland, College Park, Maryland, USA. |
| Abstrakt: |
Influenza A virus (IAV) causes significant morbidity and mortality in the human population. Tethered mucin 1 (MUC1) is highly expressed in airway epithelium, the primary site of IAV replication, and also by other cell types that influence IAV infection, including macrophages. MUC1 has the potential to influence infection dynamics through physical interactions and/or signaling activity, yet MUC1 modulation and its impact during viral pathogenesis remain unclear. Thus, we investigated MUC1-IAV interactions in an in vitro model of human airway epithelium (HAE). Our data indicate that a recombinant IAV hemagglutinin (H3) and H3N2 virus can bind endogenous HAE MUC1. Notably, infection of HAE with H1N1 or H3N2 IAV strains does not trigger MUC1 shedding but instead stimulates an increase in cell-associated MUC1 protein. We observed a similar increase after type I or III interferon (IFN) stimulation; however, inhibition of IFN signaling during H1N1 infection only partially abrogated this increase, indicating that multiple soluble factors contribute to MUC1 upregulation during the antiviral response. In addition to HAE, primary human monocyte-derived macrophages also upregulated MUC1 protein in response to IFN treatment and conditioned media from IAV-infected HAE. Then, to determine the impact of MUC1 on IAV pathogenesis, we developed HAE genetically depleted of MUC1 and found that MUC1 knockout cultures exhibited enhanced viral growth compared to control cultures for several IAV strains. Together, our data support a model whereby MUC1 inhibits productive uptake of IAV in HAE. Infection then stimulates MUC1 expression on multiple cell types through IFN-dependent and -independent mechanisms that further impact infection dynamics. IMPORTANCE Influenza A virus (IAV) targets airway epithelial cells for infection. Large, heavily glycosylated molecules known as tethered mucins extend from the airway epithelial cell surface and may physically restrict pathogen access to underlying cells. Additionally, tethered mucin 1 (MUC1) can be differentially phosphorylated based on external stimuli and can influence inflammation. Given MUC1's multifunctional capability, we sought to define its role during IAV infection. Here, we demonstrate that IAV directly interacts with MUC1 in a physiologically relevant model of human airway epithelium (HAE) and find that MUC1 protein expression is elevated throughout the epithelium and in primary human monocyte-derived macrophages in response to antiviral signals produced during infection. Using CRISPR/Cas9-modified HAE, we demonstrated more efficient IAV infection when MUC1 is genetically ablated. Our data suggest that MUC1 physically restricts IAV uptake and represents a dynamic component of the host response that acts to inhibit viral spread, yielding new insight into mucin-mediated antiviral defense. |
