New 1,3,4-oxadiazole compound with effective antibacterial and antibiofilm activity against Staphylococcus aureus.

Autor: Dante Formagio M; Postgraduate Program of Health Sciences, State University of Maringá, Maringá, Paraná, Brazil., de Oliveira Silva JV; Postgraduate Program of Health Sciences, State University of Maringá, Maringá, Paraná, Brazil., Fortunato Prohmann L; State University of Maringá, Maringá, Paraná, Brazil., Zanetti Campanerut-Sá PA; Postgraduate Program of Health Sciences, State University of Maringá, Maringá, Paraná, Brazil.; Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá, Paraná, Brazil., Grenier Capoci IR; Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá, Paraná, Brazil., Seki Kioshima Cotica É; Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá, Paraná, Brazil., Graton Mikcha JM; Postgraduate Program of Health Sciences, State University of Maringá, Maringá, Paraná, Brazil.; Department of Clinical Analysis and Biomedicine, State University of Maringá, Maringá, Paraná, Brazil.
Jazyk: angličtina
Zdroj: Letters in applied microbiology [Lett Appl Microbiol] 2022 Oct; Vol. 75 (4), pp. 957-966. Date of Electronic Publication: 2022 Jun 29.
DOI: 10.1111/lam.13766
Abstrakt: Staphylococcus aureus is one of the main aetiological agents causing food-borne diseases. Some strains produce enterotoxins responsible for food poisoning. In addition, they can form biofilms on several surfaces such as plastics, glass and stainless steel making it difficult to eliminate them. The present study evaluated, for the first time, the antibacterial and antibiofilm activities of the synthetic compound LMM6 against S. aureus. The minimum inhibitory concentration was 0·97, 1·95 and 1·95 μg ml -1 against S. aureus ATCC 25923, S. aureus 629/94 and S. aureus FRI S-6, respectively. The time-kill curves showed that 96 h treatment with LMM6 reduced approximately 4 log CFU per ml at all tested concentrations. Furthermore, LMM6 reduced S. aureus preformed biofilm by approximately 1 log CFU per cm 2 . During biofilm formation, a reduction of approximately 4 log CFU per cm 2 was observed. LMM6 also reduced biofilm biomass during (~60%) and after biofilm formation (~25 to 45%), as shown by the crystal violet assay. Based on these results, we conclude that LMM6 exhibits antibacterial and antibiofilm activity and may be an innovative synthetic molecule for controlling S. aureus.
(© 2022 Society for Applied Microbiology.)
Databáze: MEDLINE
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