| Autor: |
Ferreira PMP; Laboratory of Experimental Cancerology (LabCancer), Department of Biophysics and Physiology, Federal University of Piauí, Teresina, Brazil.; Postgraduate Program in Pharmacology, Federal University of Piauí, Teresina, Brazil.; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Brazil., Sousa IJO; Laboratory of Experimental Cancerology (LabCancer), Department of Biophysics and Physiology, Federal University of Piauí, Teresina, Brazil.; Postgraduate Program in Pharmacology, Federal University of Piauí, Teresina, Brazil., Machado KN; Department of Chemistry, Institute of Exact and Biological Sciences, Federal University of Ouro Preto, Ouro Preto, Brazil., da Silva Neto LA; Department of Chemistry, Institute of Exact and Biological Sciences, Federal University of Ouro Preto, Ouro Preto, Brazil., de Freitas MM; Laboratory of Experimental Cancerology (LabCancer), Department of Biophysics and Physiology, Federal University of Piauí, Teresina, Brazil., Dos Santos IL; Laboratory of Experimental Cancerology (LabCancer), Department of Biophysics and Physiology, Federal University of Piauí, Teresina, Brazil.; Postgraduate Program in Pharmacology, Federal University of Piauí, Teresina, Brazil., do Nascimento Rodrigues DC; Laboratory of Experimental Cancerology (LabCancer), Department of Biophysics and Physiology, Federal University of Piauí, Teresina, Brazil.; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Brazil., de Sousa RWR; Laboratory of Experimental Cancerology (LabCancer), Department of Biophysics and Physiology, Federal University of Piauí, Teresina, Brazil.; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Brazil., Dos Reis AC; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Brazil.; Laboratory of Toxicological Genetics (LapGenic), Department of Biochemistry and Pharmacology, Federal University of Piauí, Teresina, Brazil., do Nascimento MLLB; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Brazil.; Laboratory of Toxicological Genetics (LapGenic), Department of Biochemistry and Pharmacology, Federal University of Piauí, Teresina, Brazil., de Menezes APM; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Brazil.; Laboratory of Toxicological Genetics (LapGenic), Department of Biochemistry and Pharmacology, Federal University of Piauí, Teresina, Brazil., do Nascimento AM; Department of Chemistry, Institute of Exact and Biological Sciences, Federal University of Ouro Preto, Ouro Preto, Brazil., de Oliveira Ferreira JR; Center for Integrative Sciences, State University of Health Sciences of Alagoas Maceió, Brazil., Peron AP; Department of Biodiversity and Nature Conservation, Federal Technological University of Paraná, Campo Mourão, Brazil., de Castro E Sousa JM; Postgraduate Program in Pharmaceutical Sciences, Federal University of Piauí, Teresina, Brazil.; Laboratory of Toxicological Genetics (LapGenic), Department of Biochemistry and Pharmacology, Federal University of Piauí, Teresina, Brazil. |
| Abstrakt: |
Stevia urticifolia Thunb. is an underexploited herb possessing bioactive flavonoids, saponins, and terpenoids. The aim of this study was to examine the antiproliferative and toxicogenetic properties of the ethyl acetate extract from Stevia urticifolia aerial parts (EtAcSur) upon Artemia salina , erythrocytes, Allium cepa and sarcoma 180 cells and fibroblasts, as well as in vivo studies on mice to determine systemic, macroscopic, and behavioral alterations and bone marrow chromosomal damage. The assessment using A. salina larvae and mouse blood cells revealed LC 50 and EC 50 values of 68.9 and 113.6 µg/ml, respectively. Root growth and mitosis were inhibited by EtAcSur, and chromosomal aberrations were detected only at 100 μg/ml. EtAcSur exhibited potent concentration-dependent viability reduction of S180 and L-929 cells and antioxidant capacity employing ABTS • and DPPH • . No previous in vivo studies were performed before with the EtAcSur. Signals of acute toxicity were not observed at 300 mg/kg. Physiological and toxicological investigations at 25 and 50 mg/mg/day i.p. for 8 days did not markedly change body or organ relative weights, nor patterns of spontaneous locomotor and exploratory activities. In contrast, clastogenic effects on bone marrow were found at 50 mg/mg/day. EtAcSur was found to (1) produce toxicity in microcrustaceans, (2) capacity as free radical scavenger, (3) antimitotic, cytotoxic and clastogenic activties upon vegetal and mammalian cells, and (4) lethality on both tumor and normal murine cells indistinctly. In vivo damage systemic effects were not remarkable and clinical signals of toxicity were not observed, suggesting the significant pharmacological potential of S. urticifolia for the development of antineoplastic agents. Abbreviations: ABTS: 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid); DMSO: dimethylsulfoxide; DPPH: 1,1-diphenyl-2-picrylhydrazyl; EC 50 : effective concentration 50%; EtAcSur: ethyl acetate extract from Stevia urticifolia aerial parts; Hb, hemoglobin; IC 50 : inhibitory concentration 50%; LC 50 ,: lethal concentration 50%; MI: mitotic index; RBC, red blood cells; Trolox: 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid. |
