Asgard archaea shed light on the evolutionary origins of the eukaryotic ubiquitin-ESCRT machinery.

Autor: Hatano T; Centre for Mechanochemical Cell Biology, Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK., Palani S; Centre for Mechanochemical Cell Biology, Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK.; Department of Biochemistry, Indian Institute of Science, Bangalore, India., Papatziamou D; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, LA1 4YG, UK., Salzer R; MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK., Souza DP; MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK., Tamarit D; Laboratory of Microbiology, Wageningen University, 6708 WE, Wageningen, The Netherlands.; Department of Aquatic Sciences and Assessment, Swedish University of Agricultural Sciences, SE-75007, Uppsala, Sweden., Makwana M; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, LA1 4YG, UK., Potter A; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, LA1 4YG, UK., Haig A; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, LA1 4YG, UK., Xu W; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, LA1 4YG, UK., Townsend D; Department of Chemistry, Lancaster University, Lancaster, LA1 4YB, UK., Rochester D; Department of Chemistry, Lancaster University, Lancaster, LA1 4YB, UK., Bellini D; MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK., Hussain HMA; Centre for Mechanochemical Cell Biology, Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK., Ettema TJG; Laboratory of Microbiology, Wageningen University, 6708 WE, Wageningen, The Netherlands., Löwe J; MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK., Baum B; MRC Laboratory of Molecular Biology, Cambridge, CB2 0QH, UK. bbaum@mrc-lmb.cam.ac.uk., Robinson NP; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, LA1 4YG, UK. n.robinson2@lancaster.ac.uk., Balasubramanian M; Centre for Mechanochemical Cell Biology, Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK. M.K.Balasubramanian@warwick.ac.uk.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2022 Jun 13; Vol. 13 (1), pp. 3398. Date of Electronic Publication: 2022 Jun 13.
DOI: 10.1038/s41467-022-30656-2
Abstrakt: The ESCRT machinery, comprising of multiple proteins and subcomplexes, is crucial for membrane remodelling in eukaryotic cells, in processes that include ubiquitin-mediated multivesicular body formation, membrane repair, cytokinetic abscission, and virus exit from host cells. This ESCRT system appears to have simpler, ancient origins, since many archaeal species possess homologues of ESCRT-III and Vps4, the components that execute the final membrane scission reaction, where they have been shown to play roles in cytokinesis, extracellular vesicle formation and viral egress. Remarkably, metagenome assemblies of Asgard archaea, the closest known living relatives of eukaryotes, were recently shown to encode homologues of the entire cascade involved in ubiquitin-mediated membrane remodelling, including ubiquitin itself, components of the ESCRT-I and ESCRT-II subcomplexes, and ESCRT-III and Vps4. Here, we explore the phylogeny, structure, and biochemistry of Asgard homologues of the ESCRT machinery and the associated ubiquitylation system. We provide evidence for the ESCRT-I and ESCRT-II subcomplexes being involved in ubiquitin-directed recruitment of ESCRT-III, as it is in eukaryotes. Taken together, our analyses suggest a pre-eukaryotic origin for the ubiquitin-coupled ESCRT system and a likely path of ESCRT evolution via a series of gene duplication and diversification events.
(© 2022. The Author(s).)
Databáze: MEDLINE
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