Nanoparticle-Protein Interaction: Demystifying the Correlation between Protein Corona and Aggregation Phenomena.

Autor:	Ferreira LF; Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), 13083-970 Campinas, Brazil.; Programa de Pós-Graduação em Biotecnociências, Universidade Federal do ABC, 09210-580 Santo André, Brazil., Picco AS; Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicadas (INIFTA), Fac. de Cs. Exactas, Universidad Nacional de La Plata─CONICET, Boulevard 113 y 64, 1900 La Plata, Argentina., Galdino FE; Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), 13083-970 Campinas, Brazil.; Institute of Chemistry (IQ), University of Campinas (UNICAMP), P.O. Box 6154, 13083-970 Campinas, Brazil., Albuquerque LJC; Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), 13083-970 Campinas, Brazil., Berret JF; Université Paris Cité, CNRS, Matière et Systèmes Complexes, 75013 Paris, France., Cardoso MB; Brazilian Synchrotron Light Laboratory (LNLS), Brazilian Center for Research in Energy and Materials (CNPEM), 13083-970 Campinas, Brazil.; Programa de Pós-Graduação em Biotecnociências, Universidade Federal do ABC, 09210-580 Santo André, Brazil.; Institute of Chemistry (IQ), University of Campinas (UNICAMP), P.O. Box 6154, 13083-970 Campinas, Brazil.
Jazyk:	angličtina
Zdroj:	ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2022 Jun 29; Vol. 14 (25), pp. 28559-28569. Date of Electronic Publication: 2022 Jun 13.
DOI:	10.1021/acsami.2c05362
Abstrakt:	Protein corona formation and nanoparticles' aggregation have been heavily discussed over the past years since the lack of fine-mapping of these two combined effects has hindered the targeted delivery evolution and the personalized nanomedicine development. We present a multitechnique approach that combines dynamic light and small-angle X-ray scattering techniques with cryotransmission electron microscopy in a given fashion that efficiently distinguishes protein corona from aggregates formation. This methodology was tested using ∼25 nm model silica nanoparticles incubated with either model proteins or biologically relevant proteomes (such as fetal bovine serum and human plasma) in low and high ionic strength buffers to precisely tune particle-to-protein interactions. In this work, we were able to differentiate protein corona, small aggregates formation, and massive aggregation, as well as obtain fractal information on the aggregates reliably and straightforwardly. The strategy presented here can be expanded to other particle-to-protein mixtures and might be employed as a quality control platform for samples that undergo biological tests.
Databáze:	MEDLINE
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