Ultrasensitive Detection of MCF-7 Cells with a Carbon Nanotube-Based Optoelectronic-Pulse Sensor Framework.

Autor:	Chan SSY; Department of Science, Mathematics and Technology, Singapore University of Technology and Design, Singapore487372, Singapore., Lee D; Department of Science, Mathematics and Technology, Singapore University of Technology and Design, Singapore487372, Singapore., Meivita MP; Department of Science, Mathematics and Technology, Singapore University of Technology and Design, Singapore487372, Singapore., Li L; Department of Science, Mathematics and Technology, Singapore University of Technology and Design, Singapore487372, Singapore.; Thomas Young Centre and Department of Chemical Engineering, University College London, LondonWC1E 6BT, U.K., Tan YS; Bioinformatics Institute, Agency for Science, Technology and Research (ASTAR), Singapore138671, Singapore., Bajalovic N; Department of Science, Mathematics and Technology, Singapore University of Technology and Design, Singapore487372, Singapore., Loke DK; Department of Science, Mathematics and Technology, Singapore University of Technology and Design, Singapore487372, Singapore.; Office of Innovation, Changi General Hospital, Singapore529889, Singapore.
Jazyk:	angličtina
Zdroj:	ACS omega [ACS Omega] 2022 May 26; Vol. 7 (22), pp. 18459-18470. Date of Electronic Publication: 2022 May 26 (Print Publication: 2022).
DOI:	10.1021/acsomega.2c00842
Abstrakt:	Biosensors are of vital significance for healthcare by supporting the management of infectious diseases for preventing pandemics and the diagnosis of life-threatening conditions such as cancer. However, the advancement of the field can be limited by low sensing accuracy. Here, we altered the bioelectrical signatures of the cells using carbon nanotubes (CNTs) via structural loosening effects. Using an alternating current (AC) pulse under light irradiation, we developed a photo-assisted AC pulse sensor based on CNTs to differentiate between healthy breast epithelial cells (MCF-10A) and luminal breast cancer cells (MCF-7) within a heterogeneous cell population. We observed a previously undemonstrated increase in current contrast for MCF-7 cells with CNTs compared to MCF-10A cells with CNTs under light exposure. Moreover, we obtained a detection limit of ∼1.5 × 10 3 cells below a baseline of ∼1 × 10 4 cells for existing electrical-based sensors for an adherent, heterogeneous cell population. All-atom molecular dynamics (MD) simulations reveal that interactions between the embedded CNT and cancer cell membranes result in a less rigid lipid bilayer structure, which can facilitate CNT translocation for enhancing current. This as-yet unconsidered cancer cell-specific method based on the unique optoelectrical properties of CNTs represents a strategy for unlocking the detection of a small population of cancer cells and provides a promising route for the early diagnosis, monitoring, and staging of cancer. Competing Interests: The authors declare no competing financial interest. (© 2022 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
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