Isolation and comparison of neural stem cells from the adult rat brain and spinal cord canonical neurogenic niches.

Autor:	Schiro LE; Department of Neuromedicine and Movement Science, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology (NTNU), 7030 Trondheim, Norway., Bauer US; Department of Neuromedicine and Movement Science, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology (NTNU), 7030 Trondheim, Norway., Sandvig A; Department of Neuromedicine and Movement Science, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology (NTNU), 7030 Trondheim, Norway.; Department of Clinical Neuroscience, Division of Neuro, Head, and Neck, Umeå University Hospital, 907 37 Umeå, Sweden.; Department of Community and Rehabilitation, Division of Neuro, Head and Neck, Umeå University Hospital, 907 37 Umeå, Sweden., Sandvig I; Department of Neuromedicine and Movement Science, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology (NTNU), 7030 Trondheim, Norway.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2022 Jun 07; Vol. 3 (2), pp. 101426. Date of Electronic Publication: 2022 Jun 07 (Print Publication: 2022).
DOI:	10.1016/j.xpro.2022.101426
Abstrakt:	Here, we present a unified protocol for the extraction, culture, and basic characterization of rat neural stem cells (NSCs) from all three canonical neurogenic niches in the brain and spinal cord. We describe tissue dissection and dissociation, cell culture, followed by EdU labeling and characterization of NSCs. By yielding considerable numbers of viable cells per animal, this protocol enables the establishment of substantial, long-term cell banks, thus offering cost and labor efficiency while significantly reducing the numbers of animals used. Competing Interests: The authors declare no competing interests. (© 2022 The Author(s).)
Databáze:	MEDLINE
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