Application of digital PCR to determine the reliability of Xpert Xpress SARS-CoV-2 assay with envelope (E) gene negative and nucleocapsid (N2) gene positive results.

Autor: Wong RC; Department of Microbiology, Prince of Wales Hospital, Hong Kong. Electronic address: wcw372@ha.org.hk., Wong AH; Department of Microbiology, Prince of Wales Hospital, Hong Kong., Ho YI; Department of Microbiology, Prince of Wales Hospital, Hong Kong., Siu GK; Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hung Hom, Hong Kong., Lee LK; Department of Health Technology and Informatics, The Hong Kong Polytechnic University, Hung Hom, Hong Kong., Leung EC; Department of Microbiology, Prince of Wales Hospital, Hong Kong., Lai RW; Department of Microbiology, Prince of Wales Hospital, Hong Kong.
Jazyk: angličtina
Zdroj: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2022 Aug; Vol. 103 (4), pp. 115726. Date of Electronic Publication: 2022 May 20.
DOI: 10.1016/j.diagmicrobio.2022.115726
Abstrakt: This study used digital polymerase chain reaction (dPCR) to determine whether envelope (E) gene-negative and nucleocapsid (N2) gene-positive (E-N+) results obtained with the Cepheid Xpert Xpress SARS-CoV-2 assay are reliable. Using droplet digital PCR results as a reference, 18 of 22 E-N+ samples with a low viral load (81.8%) were identified as true positives.
Competing Interests: Declaration of competing interest The authors declare that we have no conflicts of interest.
(Copyright © 2022 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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