CRISPR-Cas, Argonaute proteins and the emerging landscape of amplification-free diagnostics.

Autor: Santiago-Frangos A; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA., Nemudryi A; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA., Nemudraia A; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA., Wiegand T; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA., Nichols JE; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA., Krishna P; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA., Scherffius AM; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA., Zahl TR; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA., Wilkinson RA; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA., Wiedenheft B; Department of Microbiology and Cell Biology, Montana State University, Bozeman, MT, USA. Electronic address: bwiedenheft@gmail.com.
Jazyk: angličtina
Zdroj: Methods (San Diego, Calif.) [Methods] 2022 Sep; Vol. 205, pp. 1-10. Date of Electronic Publication: 2022 Jun 09.
DOI: 10.1016/j.ymeth.2022.06.002
Abstrakt: Polymerase Chain Reaction (PCR) is the reigning gold standard for molecular diagnostics. However, the SARS-CoV-2 pandemic reveals an urgent need for new diagnostics that provide users with immediate results without complex procedures or sophisticated equipment. These new demands have stimulated a tsunami of innovations that improve turnaround times without compromising the specificity and sensitivity that has established PCR as the paragon of diagnostics. Here we briefly introduce the origins of PCR and isothermal amplification, before turning to the emergence of CRISPR-Cas and Argonaute proteins, which are being coupled to fluorimeters, spectrometers, microfluidic devices, field-effect transistors, and amperometric biosensors, for a new generation of nucleic acid-based diagnostics.
(Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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