Recent advances in microchip electrophoresis for analysis of pathogenic bacteria and viruses.

Autor: Zeid AM; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, P. R. China.; Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt., Abdussalam A; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, P. R. China.; College of Natural and Pharmaceutical Sciences, Department of Chemistry, Bayero University, Kano, Nigeria.; School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, Anhui, P. R. China., Hanif S; Department of Biological Sciences, National University of Medical Sciences (NUMS), Punjab, Pakistan., Anjum S; Department of Chemistry, Govt. Sadiq College Women University, Bahawalpur, Pakistan., Lou B; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, P. R. China.; School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, Anhui, P. R. China., Xu G; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, P. R. China.; School of Applied Chemistry and Engineering, University of Science and Technology of China, Hefei, Anhui, P. R. China.
Jazyk: angličtina
Zdroj: Electrophoresis [Electrophoresis] 2023 Jan; Vol. 44 (1-2), pp. 15-34. Date of Electronic Publication: 2022 Jun 22.
DOI: 10.1002/elps.202200082
Abstrakt: Life-threatening diseases, such as hepatitis B, pneumonia, tuberculosis, and COVID-19, are widespread due to pathogenic bacteria and viruses. Therefore, the development of highly sensitive, rapid, portable, cost-effective, and selective methods for the analysis of such microorganisms is a great challenge. Microchip electrophoresis (ME) has been widely used in recent years for the analysis of bacterial and viral pathogens in biological and environmental samples owing to its portability, simplicity, cost-effectiveness, and rapid analysis. However, microbial enrichment and purification are critical steps for accurate and sensitive analysis of pathogenic bacteria and viruses in complex matrices. Therefore, we first discussed the advances in the sample preparation technologies associated with the accurate analysis of such microorganisms, especially the on-chip microfluidic-based sample preparations such as dielectrophoresis and microfluidic membrane filtration. Thereafter, we focused on the recent advances in the lab-on-a-chip electrophoretic analysis of pathogenic bacteria and viruses in different complex matrices. As the microbial analysis is mainly based on the analysis of nucleic acid of the microorganism, the integration of nucleic acid-based amplification techniques such as polymerase chain reaction (PCR), quantitative PCR, and multiplex PCR with ME will result in an accurate and sensitive analysis of microbial pathogens. Such analyses are very important for the point-of-care diagnosis of various infectious diseases.
(© 2022 Wiley-VCH GmbH.)
Databáze: MEDLINE
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