Using the natural abundance of nitrogen isotopes to identify cattle with greater efficiency in protein-limiting diets.

Autor: Silva LFP; Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Saint Lucia, Australia. Electronic address: l.pradaesilva@uq.edu.au., Hegarty RS; The University of New England, School of Environmental and Rural Science, Armidale, Australia., Meale SJ; The University of Queensland, School of Agriculture and Food Sciences, Gatton, Australia., Costa DAF; Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Saint Lucia, Australia., Fletcher MT; Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Saint Lucia, Australia.
Jazyk: angličtina
Zdroj: Animal : an international journal of animal bioscience [Animal] 2022 Aug; Vol. 16 Suppl 3, pp. 100551. Date of Electronic Publication: 2022 Jun 07.
DOI: 10.1016/j.animal.2022.100551
Abstrakt: The difficulty in selecting cattle for higher feed and nitrogen use efficiency (NUE) is an important factor contributing to poor growth and reproductive performance in dry-tropics rangelands. Therefore, the objectives were to examine the cattle variation in retaining nitrogen in a protein-deficient diet and the natural abundance of stable isotopes in body tissues as a practical alternative for the detection of more efficient cattle. In experiment 1, feed efficiency parameters were determined in 89 Brahman steers fed a protein-limiting diet for 70 days, followed by 7 days in metabolism crates for total collection of urine and faeces and calculation of nitrogen retention and NUE. The diet-animal fractionation of nitrogen isotopes (Δ 15 N) was quantified in tail hair and plasma proteins using isotope-ratio MS. There was a large variation in growth performance, feed efficiency and nitrogen losses among steers. Quantifying Δ 15 N in tail hair (Δ 15 N tail hair ) resulted in stronger correlations with feed efficiency and nitrogen metabolism parameters than when quantified in plasma proteins. Δ 15 N tail hair was positively correlated with nitrogen losses in urine (r = 0.31, P < 0.01) and faeces (r = 0.25, P = 0.04), leading to a negative correlation with NUE (r = -0.40, P < 0.01). The group of steers with lower Δ 15 N tail hair had greater feed efficiency, lower nitrogen losses, and greater NUE. In experiment 2, for evaluation of isotope fraction as a predictor of reproductive performance, 630 Brahman-crossed cows were classified for reproductive performance for 2 years. From this group, 25 cows with poor reproductive performance and 25 cows with good reproductive performance were selected. Tail hair representing 7 months of growth were segmented and analysed for carbon (δ 13 C) and nitrogen (δ 15 N) isotope enrichment. Reproductive performance was not associated with diet selection, as there was no difference in tail hair δ 13 C between groups. However, more productive cows had lower (P < 0.05) tail hair δ 15 N during the dry season, indicating differences in N metabolism and possibly lower N losses. In addition, cows with better reproductive performance and, therefore, greater nutrient demands, had similar body condition scores and a tendency (P = 0.09) for higher live weight at the end of the trial. In conclusion, the findings of the present study confirm that nitrogen isotope fractionation in tail hair can be used as a predictor of nitrogen losses, NUE, and reproductive performance of Brahman cattle on low-protein diets.
(Copyright © 2022 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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