The immune cell atlas of human neuroblastoma.

Autor:	Verhoeven BM; Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet, 171 77 Stockholm, Sweden., Mei S; Department of Biomedical Informatics, Harvard Medical School, Boston, MA 02115, USA., Olsen TK; Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet, 171 77 Stockholm, Sweden., Gustafsson K; Center for Regenerative Medicine, Massachusetts General Hospital, Boston, MA, USA., Valind A; Department of Laboratory Medicine, Division of Clinical Genetics, Lund University, 221 85 Lund, Sweden; Department of Pediatrics, Skåne University Hospital, Lund, Sweden., Lindström A; Department of Laboratory Medicine, Division of Clinical Genetics, Lund University, 221 85 Lund, Sweden., Gisselsson D; Department of Laboratory Medicine, Division of Clinical Genetics, Lund University, 221 85 Lund, Sweden., Fard SS; Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet, 171 77 Stockholm, Sweden., Hagerling C; Department of Laboratory Medicine, Division of Clinical Genetics, Lund University, 221 85 Lund, Sweden., Kharchenko PV; Department of Biomedical Informatics, Harvard Medical School, Boston, MA 02115, USA., Kogner P; Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet, 171 77 Stockholm, Sweden., Johnsen JI; Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet, 171 77 Stockholm, Sweden., Baryawno N; Childhood Cancer Research Unit, Department of Women's and Children's Health, Karolinska Institutet, 171 77 Stockholm, Sweden. Electronic address: n.baryawno@ki.se.
Jazyk:	angličtina
Zdroj:	Cell reports. Medicine [Cell Rep Med] 2022 Jun 21; Vol. 3 (6), pp. 100657. Date of Electronic Publication: 2022 Jun 09.
DOI:	10.1016/j.xcrm.2022.100657
Abstrakt:	Understanding the complete immune cell composition of human neuroblastoma (NB) is crucial for the development of immunotherapeutics. Here, we perform single-cell RNA sequencing (scRNA-seq) on 19 human NB samples coupled with multiplex immunohistochemistry, survival analysis, and comparison with normal fetal adrenal gland data. We provide a comprehensive immune cell landscape and characterize cell-state changes from normal tissue to NB. Our analysis reveals 27 immune cell subtypes, including distinct subpopulations of myeloid, NK, B, and T cells. Several different cell types demonstrate a survival benefit. In contrast to adult cancers and previous NB studies, we show an increase in inflammatory monocyte cell state when contrasting normal and tumor tissue, while no differences in cytotoxicity and exhaustion score for T cells, nor in Treg activity, are observed. Our receptor-ligand interaction analysis reveals a highly complex interactive network of the NB microenvironment from which we highlight several interactions that we suggest for future therapeutic studies. Competing Interests: Declaration of interests P.V.K. serves on the Scientific Advisory Board to Celsius Therapeutics and Biomage and also consults for Altos Labs. (Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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