Identification of bronchoalveolar and blood immune-inflammatory biomarker signature associated with poor 28-day outcome in critically ill COVID-19 patients.

Autor: Voiriot G; Assistance Publique-Hôpitaux de Paris, Service de médecine intensive réanimation, Hôpital Tenon, Sorbonne Université, 4 rue de la Chine, 75020, Paris, France. guillaume.voiriot@aphp.fr., Dorgham K; Département d'immunologie, INSERM Centre d'Immunologie Et Des Maladies Infectieuses CIMI-Paris, Assistance Publique-Hôpitaux de Paris, Hôpital Pitié-Salpêtrière, Sorbonne Université, Paris, France., Bachelot G; Département de biochimie, INSERM Centre de Recherche Saint-Antoine (CRSA), Assistance Publique-Hôpitaux de Paris, Hôpital Saint-Antoine, Sorbonne Université, Paris, France., Fajac A; Assistance Publique-Hôpitaux de Paris, Service d'anatomie et cytologie pathologiques, Hôpital Tenon, Sorbonne Université, Paris, France., Morand-Joubert L; INSERM Institut Pierre Louis d'épidémiologie et de Santé Publique, Assistance Publique-Hôpitaux de Paris, Laboratoire de VirologieHôpital Saint-Antoine, Sorbonne Université, Paris, France., Parizot C; Département d'immunologie, INSERM Centre d'Immunologie Et Des Maladies Infectieuses CIMI-Paris, Assistance Publique-Hôpitaux de Paris, Hôpital Pitié-Salpêtrière, Sorbonne Université, Paris, France., Gerotziafas G; INSERM U938 'Cancer, Haemostasis and Angiogenesis' Centre de Recherche Saint-Antoine, Assistance Publique-Hôpitaux de Paris, Service d'hématologie biologique, Hôpital Tenon, Sorbonne Université, Paris, France., Farabos D; Département de biochimie, INSERM Centre de Recherche Saint-Antoine (CRSA), Assistance Publique-Hôpitaux de Paris, Hôpital Saint-Antoine, Sorbonne Université, Paris, France., Trugnan G; Département de biochimie, INSERM Centre de Recherche Saint-Antoine (CRSA), Assistance Publique-Hôpitaux de Paris, Hôpital Saint-Antoine, Sorbonne Université, Paris, France., Eguether T; Département de biochimie, INSERM Centre de Recherche Saint-Antoine (CRSA), Assistance Publique-Hôpitaux de Paris, Hôpital Saint-Antoine, Sorbonne Université, Paris, France., Blayau C; Assistance Publique-Hôpitaux de Paris, Service de médecine intensive réanimation, Hôpital Tenon, Sorbonne Université, 4 rue de la Chine, 75020, Paris, France., Djibré M; Assistance Publique-Hôpitaux de Paris, Service de médecine intensive réanimation, Hôpital Tenon, Sorbonne Université, 4 rue de la Chine, 75020, Paris, France., Elabbadi A; Assistance Publique-Hôpitaux de Paris, Service de médecine intensive réanimation, Hôpital Tenon, Sorbonne Université, 4 rue de la Chine, 75020, Paris, France., Gibelin A; Assistance Publique-Hôpitaux de Paris, Service de médecine intensive réanimation, Hôpital Tenon, Sorbonne Université, 4 rue de la Chine, 75020, Paris, France., Labbé V; Assistance Publique-Hôpitaux de Paris, Service de médecine intensive réanimation, Hôpital Tenon, Sorbonne Université, 4 rue de la Chine, 75020, Paris, France., Parrot A; Assistance Publique-Hôpitaux de Paris, Service de pneumologie, Hôpital Tenon, Sorbonne Université, Paris, France., Turpin M; Assistance Publique-Hôpitaux de Paris, Service de médecine intensive réanimation, Hôpital Tenon, Sorbonne Université, 4 rue de la Chine, 75020, Paris, France., Cadranel J; Assistance Publique-Hôpitaux de Paris, Service de pneumologie, Hôpital Tenon, Sorbonne Université, Paris, France., Gorochov G; Département d'immunologie, INSERM Centre d'Immunologie Et Des Maladies Infectieuses CIMI-Paris, Assistance Publique-Hôpitaux de Paris, Hôpital Pitié-Salpêtrière, Sorbonne Université, Paris, France., Fartoukh M; Assistance Publique-Hôpitaux de Paris, Service de médecine intensive réanimation, Hôpital Tenon, Sorbonne Université, 4 rue de la Chine, 75020, Paris, France., Lamazière A; Département de biochimie, INSERM Centre de Recherche Saint-Antoine (CRSA), Assistance Publique-Hôpitaux de Paris, Hôpital Saint-Antoine, Sorbonne Université, Paris, France.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2022 Jun 09; Vol. 12 (1), pp. 9502. Date of Electronic Publication: 2022 Jun 09.
DOI: 10.1038/s41598-022-13179-0
Abstrakt: The local immune-inflammatory response elicited by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is still poorly described, as well as the extent to which its characteristics may be associated with the outcome of critical Coronavirus disease 2019 (COVID-19). In this prospective monocenter study, all consecutive COVID-19 critically ill patients admitted from February to December 2020 and explored by fiberoptic bronchoscopy with bronchoalveolar lavage (BAL) were included. Biological assays, including digital ELISA cytokine profiling and targeted eicosanoid metabolomic analysis, were performed on paired blood and BAL fluid (BALF). Clinical outcome was assessed through the World Health Organization 10-point Clinical Progression Scale (WHO-CPS) at the 28th day (D28) following the admission to intensive care unit. A D28-WHO-CPS value higher than 5 defined a poor outcome. Seventy-six patients were included, 45 (59%) had a poor day-28 outcome. As compared to their counterparts, patients with D28-WHO-CPS > 5 exhibited a neutrophil-predominant bronchoalveolar phenotype, with a higher BALF neutrophil/lymphocyte ratio, a blunted local type I interferon response, a decompartimentalized immune-inflammatory response illustrated by lower BALF/blood ratio of concentrations of IL-6 (1.68 [0.30-4.41] vs. 9.53 [2.56-19.1]; p = 0.001), IL-10, IL-5, IL-22 and IFN-γ, and a biological profile of vascular endothelial injury illustrated by a higher blood concentration of VEGF and higher blood and/or BALF concentrations of several vasoactive eicosanoids. In critically ill COVID-19 patients, we identified bronchoalveolar and blood immune-inflammatory biomarker signature associated with poor 28-day outcome.
(© 2022. The Author(s).)
Databáze: MEDLINE
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