Detection of highly macrolide-resistant Legionella pneumophila strains from a hotel water network using systematic whole-genome sequencing.

Autor:	Ginevra C; Hospices Civils de Lyon, Groupement Hospitalier Nord, Institut des Agents Infectieux, Centre National de Référence des Légionelles, Lyon, France.; Centre International de Recherche en Infectiologie (CIRI), Team Legionella Pathogenesis, Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, F-69007, Lyon, France.; Hospices Civils de Lyon, Groupement Hospitalier Nord, Institut des Agents Infectieux, Plateforme GenEPII, Lyon, France., Beraud L; Hospices Civils de Lyon, Groupement Hospitalier Nord, Institut des Agents Infectieux, Centre National de Référence des Légionelles, Lyon, France., Pionnier I; Agence Régionale de Santé Auvergne-Rhône-Alpes, Lyon, France., Sallabery K; Agence Régionale de Santé Auvergne-Rhône-Alpes, Lyon, France., Bentayeb H; Centre Hospitalier Saint-Quentin, Service de Pneumologie, Saint-Quentin, France., Simon B; Hospices Civils de Lyon, Groupement Hospitalier Nord, Institut des Agents Infectieux, Plateforme GenEPII, Lyon, France., Allam C; Hospices Civils de Lyon, Groupement Hospitalier Nord, Institut des Agents Infectieux, Centre National de Référence des Légionelles, Lyon, France.; Centre International de Recherche en Infectiologie (CIRI), Team Legionella Pathogenesis, Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, F-69007, Lyon, France., Chastang J; Hospices Civils de Lyon, Groupement Hospitalier Nord, Institut des Agents Infectieux, Centre National de Référence des Légionelles, Lyon, France., Ibranosyan M; Hospices Civils de Lyon, Groupement Hospitalier Nord, Institut des Agents Infectieux, Centre National de Référence des Légionelles, Lyon, France.; Centre International de Recherche en Infectiologie (CIRI), Team Legionella Pathogenesis, Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, F-69007, Lyon, France., Decroix V; Centre Hospitalier Saint-Quentin, Laboratoire de Biologie Médicale, Saint-Quentin, France., Campese C; Santé Publique France, Unité des infections respiratoires et vaccinations, Saint-Maurice, France., Jarraud S; Hospices Civils de Lyon, Groupement Hospitalier Nord, Institut des Agents Infectieux, Centre National de Référence des Légionelles, Lyon, France.; Centre International de Recherche en Infectiologie (CIRI), Team Legionella Pathogenesis, Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, F-69007, Lyon, France.; Hospices Civils de Lyon, Groupement Hospitalier Nord, Institut des Agents Infectieux, Plateforme GenEPII, Lyon, France., Descours G; Hospices Civils de Lyon, Groupement Hospitalier Nord, Institut des Agents Infectieux, Centre National de Référence des Légionelles, Lyon, France.; Centre International de Recherche en Infectiologie (CIRI), Team Legionella Pathogenesis, Univ Lyon, Inserm, U1111, Université Claude Bernard Lyon 1, CNRS, UMR5308, ENS de Lyon, F-69007, Lyon, France.
Jazyk:	angličtina
Zdroj:	The Journal of antimicrobial chemotherapy [J Antimicrob Chemother] 2022 Jul 28; Vol. 77 (8), pp. 2167-2170.
DOI:	10.1093/jac/dkac173
Abstrakt:	Objectives: Implementation of an antibiotic resistance detection tool in Legionella daily surveillance at the French National Reference Centre for Legionella. Methods: Systematic WGS of Legionella pneumophila isolates and bioinformatics detection of specific mutations linked to antibiotic resistance. Phenotypic validation of antibiotic resistance detected by WGS was performed by the broth microdilution method. Results: More than 3000 L. pneumophila strains were screened for antibiotic resistance. A macrolide resistance-associated A2052G mutation in the 23S rRNA gene was identified in the genome of eight isolates from a hotel water network. High-level macrolide resistance (i.e. MICs of 1024-2048 mg/L for azithromycin and erythromycin) with no cross-resistance to other antimicrobials was phenotypically confirmed by antimicrobial susceptibility testing for the eight isolates. Conclusions: Systematic WGS of L. pneumophila is a powerful tool for first-line high-throughput screening of antibiotic resistance before phenotypic validation. (© The Author(s) 2022. Published by Oxford University Press on behalf of British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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