Preliminary results on a proposed histopathological assessment of predictive factors for basal cell carcinoma recurrence after primary free margin excision.

Autor: Jacquet A; Service of Pathology CHU Reims University Hospital of Reims Reims France., Dormoy V; Inserm P3Cell UMR-S1250 SFR CAP-SANTE University of Reims Champagne-Ardenne Reims France., Lorenzato M; Service of Pathology CHU Reims University Hospital of Reims Reims France., Durlach A; Service of Pathology CHU Reims University Hospital of Reims Reims France.; Inserm P3Cell UMR-S1250 SFR CAP-SANTE University of Reims Champagne-Ardenne Reims France.
Jazyk: angličtina
Zdroj: Skin health and disease [Skin Health Dis] 2022 Mar 25; Vol. 2 (2), pp. e88. Date of Electronic Publication: 2022 Mar 25 (Print Publication: 2022).
DOI: 10.1002/ski2.88
Abstrakt: Background: Basal cell carcinoma (BCC) incidence is steadily increasing but therapeutic solutions remain limited and present a public health challenge.
Aims: To identify predictive factors of BCC recurrence after primary free margin excision, with automated methods, by evaluating cell proliferation, the Hedgehog pathway activation and primary cilia.
Materials and Methods: This case-control study included 32 patients (16 with recurrence occurring at least 6 months after complete resection, and 16 without recurrence) who underwent surgery for BCC. Formalin-fixed paraffin-embedded cutaneous resections were processed for immunohistochemistry or immunostaining with the following primary antibodies: mouse anti-MCM6, rabbit anti-ARL13B and rabbit anti-GLI1.
Results: BCC recurrence after free margin excision was significantly linked to a higher proliferative index ( p  < 0.001) and a lower cilia count ( p  = 0.041) in the primary lesion. No significant differences were observed regarding cilia length ( p  = 0.39) or GLI1-positive nuclei.
Discussion: The complex interplay between essential signaling pathways, cell proliferation and cilia requires further experimental investigations in the context of BCC recurrence.
Conclusion: A higher proliferative index evaluated with MCM6 antibody could be a useful prognosis marker of BCC risk of recurrence. The lower cilia count in the primary lesion unveiled novel perspectives to understand BCC recurrence molecular mechanisms.
Competing Interests: Valérian Dormoy reports personal fees from Chiesi outside the submitted work.
(© 2021 The Authors. Skin Health and Disease published by John Wiley & Sons Ltd on behalf of British Association of Dermatologists.)
Databáze: MEDLINE
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