Comparative Analysis of Gene Expression Patterns for Oral Epithelial Cell Functions in Periodontitis.

Autor:	Gonzalez OA; Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY, United States.; Division of Periodontology, College of Dentistry, University of Kentucky, Lexington, KY, United States., Kirakodu S; Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY, United States., Nguyen LM; Department of Biomedical Sciences, School of Dental Medicine, University of Nevada Las Vegas, Las Vegas, NV, United States., Orraca L; School of Dentistry, University of Puerto Rico, San Juan, Puerto Rico., Novak MJ; Center for Oral Health Research, College of Dentistry, University of Kentucky, Lexington, KY, United States., Gonzalez-Martinez J; Department of Biomedical Sciences, School of Dental Medicine, University of Nevada Las Vegas, Las Vegas, NV, United States., Ebersole JL; Department of Biomedical Sciences, School of Dental Medicine, University of Nevada Las Vegas, Las Vegas, NV, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in oral health [Front Oral Health] 2022 May 23; Vol. 3, pp. 863231. Date of Electronic Publication: 2022 May 23 (Print Publication: 2022).
DOI:	10.3389/froh.2022.863231
Abstrakt:	The structure and function of epithelial cells are critical for the construction and maintenance of intact epithelial surfaces throughout the body. Beyond the mechanical barrier functions, epithelial cells have been identified as active participants in providing warning signals to the host immune and inflammatory cells and in communicating various detailed information on the noxious challenge to help drive specificity in the characteristics of the host response related to health or pathologic inflammation. Rhesus monkeys were used in these studies to evaluate the gingival transcriptome for naturally occurring disease samples (GeneChip® Rhesus Macaque Genome Array) or for ligature-induced disease (GeneChip® Rhesus Gene 1.0 ST Array) to explore up to 452 annotated genes related to epithelial cell structure and functions. Animals were distributed by age into four groups: ≤ 3 years (young), 3-7 years (adolescent), 12-16 years (adult), and 18-23 years (aged). For naturally occurring disease, adult and aged periodontitis animals were used, which comprised 34 animals (14 females and 20 males). Groups of nine animals in similar age groups were included in a ligature-induced periodontitis experiment. A buccal gingival sample from either healthy or periodontitis-affected tissues were collected, and microarray analysis performed. The overall results of this investigation suggested a substantial alteration in epithelial cell functions that occurs rapidly with disease initiation. Many of these changes were prolonged throughout disease progression and generally reflect a disruption of normal cellular functions that would presage the resulting tissue destruction and clinical disease measures. Finally, clinical resolution may not signify biological resolution and represent a continued risk for disease that may require considerations for additional biologically specific interventions to best manage further disease. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Gonzalez, Kirakodu, Nguyen, Orraca, Novak, Gonzalez-Martinez and Ebersole.)
Databáze:	MEDLINE
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