Broadly recognized, cross-reactive SARS-CoV-2 CD4 T cell epitopes are highly conserved across human coronaviruses and presented by common HLA alleles.

Autor:	Becerra-Artiles A; Department of Pathology, UMass Chan Medical School, Worcester, MA 01655, USA., Calvo-Calle JM; Department of Pathology, UMass Chan Medical School, Worcester, MA 01655, USA., Co MD; Department of Medicine, UMass Chan Medical School, Worcester, MA 01655, USA., Nanaware PP; Department of Pathology, UMass Chan Medical School, Worcester, MA 01655, USA., Cruz J; Department of Pathology, UMass Chan Medical School, Worcester, MA 01655, USA., Weaver GC; Department of Pathology, UMass Chan Medical School, Worcester, MA 01655, USA., Lu L; Department of Pathology, UMass Chan Medical School, Worcester, MA 01655, USA., Forconi C; Department of Medicine, UMass Chan Medical School, Worcester, MA 01655, USA., Finberg RW; Department of Medicine, UMass Chan Medical School, Worcester, MA 01655, USA., Moormann AM; Department of Medicine, UMass Chan Medical School, Worcester, MA 01655, USA., Stern LJ; Department of Pathology, UMass Chan Medical School, Worcester, MA 01655, USA; Department of Biochemistry and Molecular Biotechnology, UMass Chan Medical School, Worcester, MA 01655, USA. Electronic address: lawrence.stern@umassmed.edu.
Jazyk:	angličtina
Zdroj:	Cell reports [Cell Rep] 2022 Jun 14; Vol. 39 (11), pp. 110952. Date of Electronic Publication: 2022 May 27.
DOI:	10.1016/j.celrep.2022.110952
Abstrakt:	Sequence homology between SARS-CoV-2 and common-cold human coronaviruses (HCoVs) raises the possibility that memory responses to prior HCoV infection can affect T cell response in COVID-19. We studied T cell responses to SARS-CoV-2 and HCoVs in convalescent COVID-19 donors and identified a highly conserved SARS-CoV-2 sequence, S 811-831 , with overlapping epitopes presented by common MHC class II proteins HLA-DQ5 and HLA-DP4. These epitopes are recognized by low-abundance CD4 T cells from convalescent COVID-19 donors, mRNA vaccine recipients, and uninfected donors. TCR sequencing revealed a diverse repertoire with public TCRs. T cell cross-reactivity is driven by the high conservation across human and animal coronaviruses of T cell contact residues in both HLA-DQ5 and HLA-DP4 binding frames, with distinct patterns of HCoV cross-reactivity explained by MHC class II binding preferences and substitutions at secondary TCR contact sites. These data highlight S 811-831 as a highly conserved CD4 T cell epitope broadly recognized across human populations. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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