| Autor: |
Thiele JC; Third Institute of Physics-Biophysics, Georg August University, 37077 Göttingen, Germany., Jungblut M; Department of Biotechnology and Biophysics, Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, Germany., Helmerich DA; Department of Biotechnology and Biophysics, Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, Germany., Tsukanov R; Third Institute of Physics-Biophysics, Georg August University, 37077 Göttingen, Germany., Chizhik A; Third Institute of Physics-Biophysics, Georg August University, 37077 Göttingen, Germany., Chizhik AI; Third Institute of Physics-Biophysics, Georg August University, 37077 Göttingen, Germany., Schnermann MJ; Chemical Biology Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, MD 21702, USA., Sauer M; Department of Biotechnology and Biophysics, Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, Germany., Nevskyi O; Third Institute of Physics-Biophysics, Georg August University, 37077 Göttingen, Germany., Enderlein J; Third Institute of Physics-Biophysics, Georg August University, 37077 Göttingen, Germany.; Cluster of Excellence 'Multiscale Bioimaging: From Molecular Machines to Networks of Excitable Cells' (MBExC), Georg August University, Göttingen, Germany. |
| Abstrakt: |
Over the past two decades, super-resolution microscopy has seen a tremendous development in speed and resolution, but for most of its methods, there exists a remarkable gap between lateral and axial resolution, which is by a factor of 2 to 3 worse. One recently developed method to close this gap is metal-induced energy transfer (MIET) imaging, which achieves an axial resolution down to nanometers. It exploits the distance-dependent quenching of fluorescence when a fluorescent molecule is brought close to a metal surface. In the present manuscript, we combine the extreme axial resolution of MIET imaging with the extraordinary lateral resolution of single-molecule localization microscopy, in particular with direct stochastic optical reconstruction microscopy ( d STORM). This combination allows us to achieve isotropic three-dimensional super-resolution imaging of subcellular structures. Moreover, we used spectral demixing for implementing dual-color MIET- d STORM that allows us to image and colocalize, in three dimensions, two different cellular structures simultaneously. |
