Assessing the Roles of Potential Notch Signaling Components in Instructive and Permissive Pathways with Two Drosophila Pericardial Reporters.

Autor:	Panta M; Department of Biology, Indiana State University, Terre Haute, IN, USA.; The Center for Genomic Advocacy, Indiana State University, Terre Haute, IN, USA., Kump AJ; Department of Biology, Indiana State University, Terre Haute, IN, USA.; The Center for Genomic Advocacy, Indiana State University, Terre Haute, IN, USA.; Rich and Robin Porter Cancer Research Center, Indiana State University, Terre Haute, IN, USA., Schwab KR; Department of Biology, Indiana State University, Terre Haute, IN, USA.; The Center for Genomic Advocacy, Indiana State University, Terre Haute, IN, USA.; Rich and Robin Porter Cancer Research Center, Indiana State University, Terre Haute, IN, USA., Ahmad SM; Department of Biology, Indiana State University, Terre Haute, IN, USA. Shaad.Ahmad@indstate.edu.; The Center for Genomic Advocacy, Indiana State University, Terre Haute, IN, USA. Shaad.Ahmad@indstate.edu.; Rich and Robin Porter Cancer Research Center, Indiana State University, Terre Haute, IN, USA. Shaad.Ahmad@indstate.edu.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2472, pp. 109-130.
DOI:	10.1007/978-1-0716-2201-8_10
Abstrakt:	The highly conserved Notch signaling pathway brings about the transcriptional activation of target genes via either instructive or permissive mechanisms that depend on the identity of the specific target gene. As additional components of the Notch signaling pathway are identified, assessing whether each of these components are utilized exclusively by one of these mechanisms (and if so, which), or by both, becomes increasingly important. Using RNA interference-mediated knockdowns of the Notch component to be tested, reporters for two Notch-activated pericardial genes in Drosophila melanogaster, immunohistochemistry, and fluorescence microscopy, we describe a method to determine the type of signaling mechanism-instructive, permissive, or both-to which a particular Notch pathway component contributes. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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