Topical liquid formulation of bacteriophages for metered-dose spray delivery.

Autor: Chang RYK; Advanced Drug Delivery Group, The University of Sydney, Faculty of Medicine and Health, School of Pharmacy, Sydney, New South Wales, Australia. Electronic address: yoon.chang@sydney.edu.au., Okamoto Y; Advanced Drug Delivery Group, The University of Sydney, Faculty of Medicine and Health, School of Pharmacy, Sydney, New South Wales, Australia; Faculty of Pharmaceutical Sciences, Sojo University, Kumamoto, Japan. Electronic address: nkhymru7@gmail.com., Morales S; Phage Consulting, Sydney, New South Wales, Australia. Electronic address: morales.sandra@gmail.com., Kutter E; The Evergreen State College, Olympia, WA, USA. Electronic address: kutterB@evergreen.edu., Chan HK; Advanced Drug Delivery Group, The University of Sydney, Faculty of Medicine and Health, School of Pharmacy, Sydney, New South Wales, Australia. Electronic address: kim.chan@sydney.edu.au.
Jazyk: angličtina
Zdroj: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V [Eur J Pharm Biopharm] 2022 Aug; Vol. 177, pp. 1-8. Date of Electronic Publication: 2022 Jun 04.
DOI: 10.1016/j.ejpb.2022.05.014
Abstrakt: Bacteriophage (phage) therapy is a promising treatment strategy to combat antibiotic-resistant bacteria. Clinical reports from a century ago, as well as recent reports have revealed safety and efficacy of phage therapy for bacterial wound infections. However, the conventional liquid phage formulation and delivery platforms reported lack of dose control as it easily runs off from the infection site and it is impossible to determine total volume transfer. The aim of this study was to formulate phage liquids for topical delivery using a metered-dose spray. Two types of anti-Pseudomonas phages, PEV1 (myovirus) and PEV31 (podovirus) were formulated in 35% ethanol in water containing non-ionic polymers. The formulations were evaluated for physical properties, ease of spray, dripping upon spraying, drying time, in vitro release profiles, antibacterial activity, and storage stability. The optimized phage-polymer spray formulations were easily sprayable with minimal dripping and fast drying time. Phages were rapidly released from the formulation and inhibited the growth of Pseudomonas aeruginosa. Both PEV1 and PEV31 remained biologically stable in the optimized formulations during storage at 4 °C for eight weeks. This study showed the topical spray formulations containing non-ionic polymers in ethanol/water could be a promising and innovative therapeutic system for delivering phages.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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