TWIK-Related Acid-Sensitive Potassium Channels (TASK-1) Emerge as Contributors to Tone Regulation in Renal Arteries at Alkaline pH.
| Autor: | Shvetsova AA; Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia., Lazarenko VS; Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia., Gaynullina DK; Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia., Tarasova OS; Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia., Schubert R; Physiology, Institute of Theoretical Medicine, Faculty of Medicine, University of Augsburg, Augsburg, Germany. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in physiology [Front Physiol] 2022 May 20; Vol. 13, pp. 895863. Date of Electronic Publication: 2022 May 20 (Print Publication: 2022). |
| DOI: | 10.3389/fphys.2022.895863 |
| Abstrakt: | Aim: TASK-1 channels are established regulators of pulmonary artery tone but their contribution to the regulation of vascular tone in systemic arteries is poorly understood. We tested the hypothesis that TASK-1 channel functional impact differs among systemic vascular beds, that this is associated with differences in their expression and may increase with alkalization of the extracellular environment. Therefore, we evaluated the expression level of TASK-1 channels and their vasomotor role in mesenteric and renal arteries. Methods: Pulmonary, mesenteric and renal arteries from male Wistar rats were used for TASK-1 channel mRNA (qPCR) and protein content (Western blotting) measurements. The functional role of TASK-1 channels was studied by wire myography using the TASK-1 channel blocker AVE1231. In some experiments, the endothelium was removed with a rat whisker. Results: Expression levels of both mRNA and protein of the TASK-1 channel pore-forming subunit were highest in pulmonary arteries, lowest in mesenteric arteries and had an intermediate value in renal arteries. Blockade of TASK-1 channels by 1 µM AVE1231 increased U46619-induced contractile responses of pulmonary arteries but did not affect basal tone and contractile responses to methoxamine of mesenteric and renal arteries at physiological extracellular pH (pHo = 7.41). At alkaline extracellular pH = 7.75 (increase of NaHCO Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Shvetsova, Lazarenko, Gaynullina, Tarasova and Schubert.) |
| Databáze: | MEDLINE |
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