Tapered fibertrodes for optoelectrical neural interfacing in small brain volumes with reduced artefacts.

Autor: Spagnolo B; Istituto Italiano di Tecnologia, CBN, Lecce, Italy. barbara.spagnolo@iit.it., Balena A; Istituto Italiano di Tecnologia, CBN, Lecce, Italy. antonio.balena@iit.it., Peixoto RT; Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, Boston, MA, USA.; Department of Psychiatry, University of Pittsburgh, Pittsburgh, PA, USA., Pisanello M; Istituto Italiano di Tecnologia, CBN, Lecce, Italy., Sileo L; Istituto Italiano di Tecnologia, CBN, Lecce, Italy., Bianco M; Istituto Italiano di Tecnologia, CBN, Lecce, Italy.; Dipartimento di Ingegneria dell'Innovazione, Università del Salento, Lecce, Italy., Rizzo A; Istituto Italiano di Tecnologia, CBN, Lecce, Italy.; Dipartimento di Ingegneria dell'Innovazione, Università del Salento, Lecce, Italy., Pisano F; Istituto Italiano di Tecnologia, CBN, Lecce, Italy., Qualtieri A; Istituto Italiano di Tecnologia, CBN, Lecce, Italy., Lofrumento DD; DiSTeBA - Department of Biological and Environmental Sciences and Technologies, Università del Salento, Lecce, Italy., De Nuccio F; DiSTeBA - Department of Biological and Environmental Sciences and Technologies, Università del Salento, Lecce, Italy., Assad JA; Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, Boston, MA, USA.; Istituto Italiano di Tecnologia, Genova, Italy., Sabatini BL; Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, Boston, MA, USA., De Vittorio M; Istituto Italiano di Tecnologia, CBN, Lecce, Italy. massimo.devittorio@iit.it.; Dipartimento di Ingegneria dell'Innovazione, Università del Salento, Lecce, Italy. massimo.devittorio@iit.it., Pisanello F; Istituto Italiano di Tecnologia, CBN, Lecce, Italy. ferruccio.pisanello@iit.it.
Jazyk: angličtina
Zdroj: Nature materials [Nat Mater] 2022 Jul; Vol. 21 (7), pp. 826-835. Date of Electronic Publication: 2022 Jun 06.
DOI: 10.1038/s41563-022-01272-8
Abstrakt: Deciphering the neural patterns underlying brain functions is essential to understanding how neurons are organized into networks. This deciphering has been greatly facilitated by optogenetics and its combination with optoelectronic devices to control neural activity with millisecond temporal resolution and cell type specificity. However, targeting small brain volumes causes photoelectric artefacts, in particular when light emission and recording sites are close to each other. We take advantage of the photonic properties of tapered fibres to develop integrated 'fibertrodes' able to optically activate small brain volumes with abated photoelectric noise. Electrodes are positioned very close to light emitting points by non-planar microfabrication, with angled light emission allowing the simultaneous optogenetic manipulation and electrical read-out of one to three neurons, with no photoelectric artefacts, in vivo. The unconventional implementation of two-photon polymerization on the curved taper edge enables the fabrication of recoding sites all around the implant, making fibertrodes a promising complement to planar microimplants.
(© 2022. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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