Generation of a human iPSC line (MPIi008-A) from a patient with Denys-Drash syndrome.

Autor:	Doeser MC; Department of Nephrology and Rheumatology, Internal Medicine D, University Hospital Münster, Albert-Schweitzer-Campus 1, Münster 48149, Germany; Interdisciplinary Center for Clinical Research (IZKF), University of Münster, Albert-Schweitzer-Campus 1, Münster 48149, Germany., Krygin J; Department of Nephrology and Rheumatology, Internal Medicine D, University Hospital Münster, Albert-Schweitzer-Campus 1, Münster 48149, Germany., Röpke A; Institute of Human Genetics, University of Münster, Vesaliusweg 12-14, Münster 48149, Germany., Han D; Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, Münster 48149, Germany., Wedlich-Söldner R; Institute of Cell Dynamics and Imaging, University of Münster, Münster 48149, Germany; Medical Faculty, University of Münster, Domagkstrasse 3, Münster 48149, Germany., Schöler HR; Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, Münster 48149, Germany; Medical Faculty, University of Münster, Domagkstrasse 3, Münster 48149, Germany., Pavenstädt H; Department of Nephrology and Rheumatology, Internal Medicine D, University Hospital Münster, Albert-Schweitzer-Campus 1, Münster 48149, Germany; Medical Faculty, University of Münster, Domagkstrasse 3, Münster 48149, Germany., Kim KP; Department of Cell and Developmental Biology, Max Planck Institute for Molecular Biomedicine, Röntgenstrasse 20, Münster 48149, Germany; Department of Medical Life Sciences, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul, Republic of Korea. Electronic address: kpkim@catholic.ac.kr.
Jazyk:	angličtina
Zdroj:	Stem cell research [Stem Cell Res] 2022 Jul; Vol. 62, pp. 102826. Date of Electronic Publication: 2022 May 30.
DOI:	10.1016/j.scr.2022.102826
Abstrakt:	An induced pluripotent stem cell (hiPSC) line (MPIi008-A) was generated from fibroblasts of a 1-year-old male patient with Denys-Drash syndrome using lentiviral delivery of reprogramming factors OCT4, SOX2, KLF4 and c-MYC. The MPIi008-A iPSC line exhibited typical iPSC morphology and normal karyotype, expressed pluripotent stem cell markers, and showed developmental potential to differentiate into derivatives of all three germ layers in vivo. The hiPSC line harbours a heterozygous missense mutation (R394L) in exon 9 of the WT1 gene. (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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