| Autor: |
Du X; State Key Laboratory of Microbial Technology, Institute of Microbiology Technology, Shandong University, Qingdao 266237, PR China., Ran Q; State Key Laboratory of Microbial Technology, Institute of Microbiology Technology, Shandong University, Qingdao 266237, PR China., Wang J; State Key Laboratory of Microbial Technology, Institute of Microbiology Technology, Shandong University, Qingdao 266237, PR China., Jiang H; College of Food Science and Engineering, Ocean University of China, 266003 Qingdao, PR China., Wang J; State Key Laboratory of Microbial Technology, Institute of Microbiology Technology, Shandong University, Qingdao 266237, PR China., Li YZ; State Key Laboratory of Microbial Technology, Institute of Microbiology Technology, Shandong University, Qingdao 266237, PR China. |
| Abstrakt: |
Two Gram-negative, rod-shaped, non-spore-forming bacteria, designated SM9 T and SM2 T , were isolated from Taklamakan Desert soil samples. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strains SM9 T and SM2 T had the highest sequence similarity to the type strains Microvirga indica BCRC 80972 T and Microvirga soli NBRC 112417 T with similarity values of 98.2 and 97.7 %, respectively, and Microvirga was among the predominant genera in the desert soil. The draft genomes of these two strains were 4.56 Mbp (SM9 T ) and 5.08 Mbp (SM2 T ) long with 65.1 mol% (SM9 T ) and 63.5 mol% (SM2 T ) G+C content. To adapt to the desert environment, these two strains possessed pathways for the synthesis of stress metabolite trehalose. The major fatty acids (>5 %) included C 18 : 1 ω9 c in SM2 T , but C 16 : 0 , C 18 : 0 and C 19 : 0 cyclo ω8 c in SM9 T , while the major menaquinone was ubiquinone 10 in both strains. The major polar lipids of SM9 T and SM2 T were phosphatidylglycerol, phosphatidylethanolamine and phospholipid. The average nucleotide identity and digital DNA-DNA hybridization results further indicated that strains SM9 T and SM2 T were distinguished from phylogenetically related species and represented two novel species within the genus Microvirga , for which the names Microvirga roseola sp. nov. (type strain SM2 T =KCTC 72792 T =CGMCC 1.17776 T ) and Microvirga lenta sp. nov. (type strain SM9 T =KCTC 82729 T =CCTCC AB 2021131 T ) are proposed. |
