RNA Design Principles for Riboswitches that Regulate RNase P-Mediated tRNA Processing.

Autor:	Ender A; Institute for Biochemistry, Leipzig University, Leipzig, Germany., Stadler PF; Bioinformatics Group, Department of Computer Science and Interdisciplinary Center for Bioinformatics, Leipzig University, Leipzig, Germany.; Max Planck Institute for Mathematics in the Science, Leipzig, Germany.; Institute for Theoretical Chemistry, University of Vienna, Vienna, Austria.; Santa Fe Institute, Santa Fe, NM, USA., Mörl M; Institute for Biochemistry, Leipzig University, Leipzig, Germany., Findeiß S; Bioinformatics Group, Department of Computer Science and Interdisciplinary Center for Bioinformatics, Leipzig University, Leipzig, Germany. sven@bioinf.uni-leipzig.de.
Jazyk:	angličtina
Zdroj:	Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2022; Vol. 2518, pp. 179-202.
DOI:	10.1007/978-1-0716-2421-0_11
Abstrakt:	Riboswitches are an attractive target for the directed design of RNA-based regulators by in silico prediction. These noncoding RNA elements consist of an aptamer platform for the highly selective ligand recognition and an expression platform which controls gene activity typically at the level of transcription or translation. In previous work, we could successfully apply RNA folding prediction to implement a new riboswitch mechanism regulating processing of a tRNA by RNase P. In this contribution, we present detailed information about our pipeline consisting of in silico design combined with the biochemical analysis for the verification of the implemented mechanism. Furthermore, we discuss the applicability of the presented biochemical in vivo and in vitro methods for the characterization of other artificial riboswitches. (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze:	MEDLINE
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