Isolation, cultivation, and application of primary respiratory epithelial cells obtained by nasal brushing, polyp samples, or lung explants.

Autor:	Golec A; Institut Necker Enfants Malades, Faculté de Médecine Necker, INSERM U1151, Université de Paris, 160 rue de Vaugirard, 75015 Paris, France., Pranke I; Institut Necker Enfants Malades, Faculté de Médecine Necker, INSERM U1151, Université de Paris, 160 rue de Vaugirard, 75015 Paris, France., Scudieri P; Medical Genetics Unit, Istituto Giannina Gaslini, 16147 Genova, Italy.; Department of Neurosciences, Rehabilitation, Ophthalmology, Genetics, Maternal and Child Health (DiNOGMI), University of Genova, 16147 Genova, Italy., Hayes K; Northern Ireland Clinical Research Facility, The Wellcome-Wolfson Institute of Experimental Medicine, Queen's University, U Floor, City Hospital, Lisburn Road, Belfast, Northern Ireland.; European Cystic Fibrosis Society, Karup, Denmark., Dreano E; Institut Necker Enfants Malades, Faculté de Médecine Necker, INSERM U1151, Université de Paris, 160 rue de Vaugirard, 75015 Paris, France., Dunlevy F; European Cystic Fibrosis Society, Karup, Denmark., Hatton A; Institut Necker Enfants Malades, Faculté de Médecine Necker, INSERM U1151, Université de Paris, 160 rue de Vaugirard, 75015 Paris, France., Downey DG; The Wellcome-Wolfson Institute of Experimental Medicine, Queen's University, Lisburn Road, Belfast, Northern Ireland., Galietta L; Telethon Institute of Genetics and Medicine (TIGEM), 80078 Pozzuoli, Napoli, Italy.; Department of Translational Medical Sciences, Università di Napoli 'Federico II,' 80131 Napoli, Italy., Sermet I; Service de Pneumologie et Allergologie Pédiatriques, Centre de Ressourcés et de Compétence de la Mucoviscidose, Hôpital Necker Enfants Malades, 149 rue de Sévres, Paris, France.; INSERM U1151, Institut Necker Enfants Malades, Université de Paris, 160 rue de Vaugirard, 75015 Paris, France.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2022 May 27; Vol. 3 (2), pp. 101419. Date of Electronic Publication: 2022 May 27 (Print Publication: 2022).
DOI:	10.1016/j.xpro.2022.101419
Abstrakt:	Here, we present a standardized protocol for isolation, maintenance, and polarization of the respiratory epithelial primary cells from patient samples acquired from nasal brushing, polyp specimens, or lung explants. This protocol generates a clearly defined polarized layer of epithelial cells on filters, with a good number of ciliated cells and a thin layer of mucus. We detail the steps for samples prepared from patients with cystic fibrosis as well as from subjects without cystic fibrosis. Competing Interests: Damian Downey has received consultancy fees and/or grant support from Vertex pharmaceuticals, Proteostasis Therapeutics, Gilead, Chiesi, and Insmed. He is Director of the European CF Society – Clinical Trials Network and a member of the UK CF Trust Research Scientific Oversight Board of the Clinical Trials Accelerator Platform. Isabelle Sermet has received consultancy fees and/or grant support from Vertex Therapeutics, Eloxx, and PTC Therapeutics and is a Board Member of the European Cystic Fibrosis Society. Luis Galietta is a member of the Advisory Board of Enterprise Therapeutics. (© 2022 The Author(s).)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu