Qualitative and Quantitative Evaluation of Donor Corneal Tissue by Slit Lamp and Specular Microscopy.

Autor:	Patil M; Ophthalmology, Dr. D. Y. Patil Medical College, Hospital & Research Centre, Pune, IND., Lune A; Ophthalmology, Dr. D. Y. Patil Medical College, Hospital & Research Centre, Pune, IND., Paranjape R; Ophthalmology, Dr. D. Y. Patil Medical College, Hospital & Research Centre, Pune, IND., Naik K; Ophthalmology, Dr. D. Y. Patil Medical College, Hospital & Research Centre, Pune, IND., Padmakumar V; Ophthalmology, Dr. D. Y. Patil Medical College, Hospital & Research Centre, Pune, IND., Alapati A; Ophthalmology, Dr. D. Y. Patil Medical College, Hospital & Research Centre, Pune, IND., Motwani D; Ophthalmology: Pediatrics, L V Prasad Eye Institute, Hyderabad, IND., Ahuja A; Ophthalmology, Dr. D. Y. Patil Medical College, Hospital & Research Centre, Pune, IND., Dhore N; Ophthalmology, Dr. D. Y. Patil Medical College, Hospital & Research Centre, Pune, IND., Kaul S; Ophthalmology, L V Prasad Eye Institute, Hyderabad, IND.
Jazyk:	angličtina
Zdroj:	Cureus [Cureus] 2022 May 03; Vol. 14 (5), pp. e24700. Date of Electronic Publication: 2022 May 03 (Print Publication: 2022).
DOI:	10.7759/cureus.24700
Abstrakt:	Background In India, donor eye collection and promotion of eye banking are insufficient to meet the needs. By adequately evaluating donor corneas, eye banks can maximize the number of viable corneas for transplantation. This study evaluated donor corneal tissue based on age, lens status, and cause of death by their morphology and endothelial cell count via slit lamp and specular microscopy. Methods We conducted a prospective observational study of all eye bank donor corneas indicated for eye donation at a tertiary hospital and research center in Western Maharashtra between September 2019 to December 2021. We evaluated the corneoscleral discs by slit-lamp microscopy specular microscopy. We analyzed donor corneas quantitatively and qualitatively and graded them accordingly. We also collected blood samples for serological testing and the donor's behavioral and family medical histories. Results We collected 94 eyes from 47 donors; the mean age of the donor population was 48.2 years, and most donors were aged 41 to 80 years. Thirty-one donors (65.96%) were male, and 16 were female (34.04%. For preservation, we used Cornisol (Aurolab, Madurai, India) in 36 cases (77%) and McCarey-Kaufman medium in 11 cases (23%). We found a mean endothelial cell density (ECD) of 2214.40/mm2, with hexagonality of 53.05%, and a coefficient of variation of 38.01. Further, we observed that ECD and hexagonality of cells in phakic donors were significantly greater than that of pseudophakic (PP) donors. Moreover, ECD and hexagonality significantly decreased in donors with the chronic disease compared to those who had a sudden, unexpected death. Conclusion Corneal grafts from younger donors, phakic donors, and donors who experienced an acute cause of death were qualitatively and quantitatively significantly better than those of older donors, PP donors, and donors who experienced sudden, unexpected death. Therefore, eye bank specular examination can improve tissue utilization and transplantation success. Therefore, we strongly recommend that eye bank personnel evaluate their donor tissue with a specular microscope to enhance the quality of eye care. Competing Interests: The authors have declared that no competing interests exist. (Copyright © 2022, Patil et al.)
Databáze:	MEDLINE
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