Effect of Surface Porosity on SARS-CoV-2 Fomite Infectivity.

Autor:	Hosseini M; Deptartment of Chemical Engineering and Center for Soft Matter and Biological Physics, Virginia Tech, Blacksburg, Virginia 24061, United States., Poon LLM; School of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, China.; Centre for Immunity and Infection, Hong Kong Science Park, Hong Kong, China.; HKU Pasteur Research Pole, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, China., Chin AWH; School of Public Health, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, China.; Centre for Immunity and Infection, Hong Kong Science Park, Hong Kong, China., Ducker WA; Deptartment of Chemical Engineering and Center for Soft Matter and Biological Physics, Virginia Tech, Blacksburg, Virginia 24061, United States.
Jazyk:	angličtina
Zdroj:	ACS omega [ACS Omega] 2022 May 23; Vol. 7 (22), pp. 18238-18246. Date of Electronic Publication: 2022 May 23 (Print Publication: 2022).
DOI:	10.1021/acsomega.1c06880
Abstrakt:	Previous reports indicated the low stability of severe actute respiratory syndrome coronovirus 2 (SARS-CoV-2) on various porous surfaces, but the role of porosity was unclear because there was no direct comparison between porous and nonporous solids of the same chemistry. Through comparing pairs of solids with very similar chemistry, we find that porosity is important: porous glass has a much lower infectivity than nonporous glass. However, porosity is not sufficient to lower infectivity; permeability, which is the ability of a liquid to move through a material, is the important parameter. We show this by comparing a pair of porous CuO coatings where the pores are accessible in one case and inaccessible in the other case. When the pores are inaccessible, the infectivity remains similar to that for nonporous solids. Thus, for both glass and CuO, it is the access to porosity that decreases the infectivity of extracted liquid droplets. Having established the importance of permeability, there is the open question of the mechanism of changing the infectivity of SARS-CoV-2. Several hypotheses are possible, such as increasing the difficulty of extracting the virus from the solid, changing the drying time, increasing the surface area of active ingredient, etc. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) measurements show that less viral DNA is extracted from a permeable surface, suggesting that the virus becomes trapped in the pores. Finally, we consider the effect of drying. We show that permeability and the water contact angle on the solid have effects on the drying time of a contaminated droplet, which may in turn affect infectivity. Competing Interests: The authors declare the following competing financial interest(s): W.D. declares part ownership in a startup company that intends to produce surface coatings. Other authors declare no conflict of interest. (© 2022 The Authors. Published by American Chemical Society.)
Databáze:	MEDLINE
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