Marker-Independent Monitoring of in vitro and in vivo Degradation of Supramolecular Polymers Applied in Cardiovascular in situ Tissue Engineering.

Autor:	Marzi J; Department for Medical Technologies and Regenerative Medicine, Institute of Biomedical Engineering, Eberhard Karls University Tübingen, Tübingen, Germany.; NMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany.; Cluster of Excellence iFIT (EXC 2180) 'Image-Guided and Functionally Instructed Tumor Therapies, ' Eberhard Karls University Tübingen, Tübingen, Germany., Munnig Schmidt EC; Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, Netherlands., Brauchle EM; Department for Medical Technologies and Regenerative Medicine, Institute of Biomedical Engineering, Eberhard Karls University Tübingen, Tübingen, Germany.; NMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany.; Cluster of Excellence iFIT (EXC 2180) 'Image-Guided and Functionally Instructed Tumor Therapies, ' Eberhard Karls University Tübingen, Tübingen, Germany., Wissing TB; Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, Netherlands.; Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology Eindhoven, Netherlands., Bauer H; Xeltis BV, Eindhoven, Netherlands., Serrero A; Xeltis BV, Eindhoven, Netherlands., Söntjens SHM; SyMO-Chem BV, Eindhoven, Netherlands., Bosman AW; SupraPolix BV, Eindhoven, Netherlands., Cox MAJ; Xeltis BV, Eindhoven, Netherlands., Smits AIPM; Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, Netherlands.; Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology Eindhoven, Netherlands., Schenke-Layland K; Department for Medical Technologies and Regenerative Medicine, Institute of Biomedical Engineering, Eberhard Karls University Tübingen, Tübingen, Germany.; NMI Natural and Medical Sciences Institute at the University of Tübingen, Reutlingen, Germany.; Cluster of Excellence iFIT (EXC 2180) 'Image-Guided and Functionally Instructed Tumor Therapies, ' Eberhard Karls University Tübingen, Tübingen, Germany.; Cardiovascular Research Laboratories, Department of Medicine, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in cardiovascular medicine [Front Cardiovasc Med] 2022 May 17; Vol. 9, pp. 885873. Date of Electronic Publication: 2022 May 17 (Print Publication: 2022).
DOI:	10.3389/fcvm.2022.885873
Abstrakt:	The equilibrium between scaffold degradation and neotissue formation, is highly essential for in situ tissue engineering. Herein, biodegradable grafts function as temporal roadmap to guide regeneration. The ability to monitor and understand the dynamics of degradation and tissue deposition in in situ cardiovascular graft materials is therefore of great value to accelerate the implementation of safe and sustainable tissue-engineered vascular grafts (TEVGs) as a substitute for conventional prosthetic grafts. In this study, we investigated the potential of Raman microspectroscopy and Raman imaging to monitor degradation kinetics of supramolecular polymers, which are employed as degradable scaffolds in in situ tissue engineering. Raman imaging was applied on in vitro degraded polymers, investigating two different polymer materials, subjected to oxidative and enzymatically-induced degradation. Furthermore, the method was transferred to analyze in vivo degradation of tissue-engineered carotid grafts after 6 and 12 months in a sheep model. Multivariate data analysis allowed to trace degradation and to compare the data from in vitro and in vivo degradation, indicating similar molecular observations in spectral signatures between implants and oxidative in vitro degradation. In vivo degradation appeared to be dominated by oxidative pathways. Furthermore, information on collagen deposition and composition could simultaneously be obtained from the same image scans. Our results demonstrate the sensitivity of Raman microspectroscopy to determine degradation stages and the assigned molecular changes non-destructively, encouraging future exploration of this techniques for time-resolved quality assessment of in situ tissue engineering processes. Competing Interests: The research labs from KS-L and AIS performed independent scientific contract work for the company Xeltis and received for this work financial compensation. MC, HB, and AS are employees of Xeltis. MC is shareholder of Xeltis and AB is a financially compensated scientific advisor to Xeltis. SS is an employee of SyMO-Chem BV. AB is an employee and shareholder in SupraPolix BV. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Marzi, Munnig Schmidt, Brauchle, Wissing, Bauer, Serrero, Söntjens, Bosman, Cox, Smits and Schenke-Layland.)
Databáze:	MEDLINE
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